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Nubsella zeaxanthinifaciens gen. nov., sp. nov., a zeaxanthin-producing bacterium of the family Sphingobacteriaceae isolated from freshwater

¹ Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA 01003, USA
² Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan

Correspondence
Dalal Asker
dasker10{at}gmail.com

A yellow-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium (TDMA-5^T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). The DNA G+C content was 38.6 mol%. Major fatty acids were iso-C_{15 : 0}, iso-C_{17 : 0} 3-OH and summed feature 4 (iso-C_{15 : 0} 2-OH and/or C_{16 : 1}7c). MK-7 was the predominant respiratory quinone. Zeaxanthin was the major carotenoid pigment produced; flexirubin-type pigments were not produced. TDMA-5^T was sensitive to gamma-irradiation. The strain degraded gelatin, casein, starch, Tween 80 and DNA. Phylogenetic analysis based on the 16S rRNA gene sequence placed TDMA-5^T in a distinct lineage in the family Sphingobacteriaceae, sharing 89.4–93.4 % sequence similarity with members of the nearest genus Pedobacter. Strain TDMA-5^T could be distinguished from the other members of the family Sphingobacteriaceae by a number of chemotaxonomic and phenotypic characteristics. Based on its unique phenotypic, genotypic and phylogenetic features, strain TDMA-5^T represents a novel genus and species, for which the name Nubsella zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain of Nubsella zeaxanthinifaciens is TDMA-5^T (=NBRC 102579^T =CCUG 54348^T).

Maximum-likelihood and parsimony phylogenetic trees based on 16S rRNA gene sequences, a scanning electron micrograph of cells of strain TDMA-5^T and a detailed fatty acid profile are available as supplementary material with the online version of this paper.