HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Syed, D. G. Articles by Li, W.-J. PubMed PubMed Citation Articles by Syed, D. G. Articles by Li, W.-J. Agricola Articles by Syed, D. G. Articles by Li, W.-J.

Saccharomonospora saliphila sp. nov., a halophilic actinomycete from an Indian soil

¹ The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China
² Department of Studies and Research in Microbiology, Gulbarga University, Gulbarga – 585 106, Karnataka, India
³ Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Korea

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
Dayanand Agasar
iamdaya62{at}rediffmail.com

A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502^T, was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 °C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H₄), while MK-8(H₄) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C_{16 : 0} (49.2 %) and C_{17 : 1}6c (9.1 %). The DNA G+C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora. 16S rRNA gene sequence similarity between strain YIM 90502^T and the type strains of Saccharomonospora species ranged from 92.42 % (with Saccharomonospora xinjiangensis CCTCC AA 97021^T) to 97.45 % (with Saccharomonospora azurea KCTC 9693^T). Levels of DNA–DNA relatedness between strain YIM 90502^T and S. azurea KCTC 9693^T, Saccharomonospora halophila DSM 44411^T and Saccharomonospora paurometabolica DSM 44619^T were 46.0, 41.0 and 42.5 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502^T was classified in the genus Saccharomonospora as a member of a novel species, for which the name Saccharomonospora saliphila sp. nov. is proposed, with YIM 90502^T (=KCTC 19234^T =DSM 45087^T) as the type strain.

A scanning electron micrograph of spores of strain YIM 90502^T and an extended 16S rRNA gene sequence-based phylogenetic tree are available as supplementary material with the online version of this paper.