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Int J Syst Evol Microbiol 58 (2008), 414-419; DOI  10.1099/ijs.0.65375-0
© 2008 International Union of Microbiological Societies

Collimonas arenae sp. nov. and Collimonas pratensis sp. nov., isolated from (semi-)natural grassland soils

S. Höppener-Ogawa1, W. de Boer1, J. H. J. Leveau1, J. A. van Veen1, E. de Brandt2, E. Vanlaere2, H. Sutton3, D. J. Dare3 and P. Vandamme2

1 Netherlands Institute of Ecology (NIOO-KNAW), Centre for Terrestrial Ecology, Boterhoeksestraat 48, 6666 GA Heteren, The Netherlands
2 Laboratory for Microbiology, Ghent University, B-9000 Ghent, Belgium
3 Manchester Metropolitan University, The Heath Business & Technical Park, Runcorn, Cheshire, UK

Correspondence
W. de Boer
w.deboer{at}nioo.knaw.nl

A polyphasic taxonomic study was performed to compare 26 novel bacterial isolates obtained from (semi-)natural grassland soils and a heathland soil in the Netherlands with 16 strains that had previously been assigned to the genus Collimonas. Genomic fingerprinting (BOX-PCR), whole-cell protein electrophoresis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry of intact cells and physiological characterization (Biolog) of the isolates confirmed the existence of different strain clusters (A–D) within the genus Collimonas. Until now, only cluster C strains have been formally classified, as Collimonas fungivorans. In this study, DNA–DNA hybridizations were performed with a selection of strains representing the four clusters. The results showed that cluster B strains also belong to C. fungivorans and that strains of clusters A and D represent two novel species within the genus Collimonas. The latter novel species could be differentiated by means of phenotypic and genotypic characteristics and are classified as Collimonas arenae sp. nov. (cluster A; type strain Ter10T =LMG 23964T =CCUG 54727T) and Collimonas pratensis sp. nov. (cluster D; type strain Ter91T =LMG 23965T =CCUG 54728T).

Abbreviations: MALDI-TOF, matrix-assisted laser desorption ionization time-of-flight

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LMG 23964T and LMG 23965T are AY281146 and AY281137, respectively. Those for other strains included in this study are listed in Table 1.

Detailed DNA–DNA hybridization results are available as supplementary material with the online version of this paper.






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