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Int J Syst Evol Microbiol 58 (2008), 319-323; DOI  10.1099/ijs.0.65238-0
© 2008 International Union of Microbiological Societies

Paenibacillus forsythiae sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of Forsythia mira

Yu-Chao Ma1,2 and San-Feng Chen1,3

1 National Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, PR China
2 Key Laboratory of Agro-Microbial and Application, China Agricultural University, Beijing 100094, PR China
3 College of Biological Sciences, China Agricultural University, Beijing 100094, PR China

Correspondence
San-Feng Chen
chensf{at}cau.edu.cn

A nitrogen-fixing bacterium, designated strain T98T, was isolated from rhizosphere soil of Forsythia mira. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain T98T was a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain T98T and Paenibacillus durus ATCC 35681T (97.0 %), Paenibacillus sabinae DSM 17841T (98.3 %) and Paenibacillus zanthoxyli DSM 18202T (96.8 %). Levels of 16S rRNA gene sequence similarity between strain T98T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain T98T and P. durus ATCC 35681T, P. sabinae DSM 17841T and P. zanthoxyli DSM 18202T were 27.6, 30.0 and 32.1 %, respectively. The DNA G+C content of strain T98T was 50.4 mol%. The major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain T98T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus forsythiae sp. nov. is proposed. The type strain is T98T (=CCBAU 10203T =DSM 17842T).

The GenBank/EMBL/DDBJ accession numbers for the partial nifH gene sequence and 16S rRNA gene sequence of strain T98T are respectively DQ349124 and DQ338443.

A neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic position of strain T98T compared with all recognized species of the genus Paenibacillus is available as supplementary material with the online version of this paper.






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