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Paenibacillus forsythiae sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of Forsythia mira

¹ National Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, PR China
² Key Laboratory of Agro-Microbial and Application, China Agricultural University, Beijing 100094, PR China
³ College of Biological Sciences, China Agricultural University, Beijing 100094, PR China

Correspondence
San-Feng Chen
chensf{at}cau.edu.cn

A nitrogen-fixing bacterium, designated strain T98^T, was isolated from rhizosphere soil of Forsythia mira. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain T98^T was a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain T98^T and Paenibacillus durus ATCC 35681^T (97.0 %), Paenibacillus sabinae DSM 17841^T (98.3 %) and Paenibacillus zanthoxyli DSM 18202^T (96.8 %). Levels of 16S rRNA gene sequence similarity between strain T98^T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain T98^T and P. durus ATCC 35681^T, P. sabinae DSM 17841^T and P. zanthoxyli DSM 18202^T were 27.6, 30.0 and 32.1 %, respectively. The DNA G+C content of strain T98^T was 50.4 mol%. The major fatty acids were anteiso-C_{15 : 0}, C_{16 : 0} and iso-C_{16 : 0}. On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain T98^T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus forsythiae sp. nov. is proposed. The type strain is T98^T (=CCBAU 10203^T =DSM 17842^T).

A neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic position of strain T98^T compared with all recognized species of the genus Paenibacillus is available as supplementary material with the online version of this paper.

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