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Int J Syst Evol Microbiol 58 (2008), 2935-2938; DOI  10.1099/ijs.0.2008/000182-0
© 2008 International Union of Microbiological Societies

Ammonifex thiophilus sp. nov., a hyperthermophilic anaerobic bacterium from a Kamchatka hot spring

Margarita L. Miroshnichenko1, Tatyana P. Tourova1, Tatyana V. Kolganova2, Nadezhda A. Kostrikina1, Nikolay Chernych1 and Elizaveta A. Bonch-Osmolovskaya1

1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia
2 Bioengineering Center, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/1, 117312 Moscow, Russia

Correspondence
Margarita Miroshnichenko
alfamirr{at}mail.ru

A novel anaerobic, extremely thermophilic, facultatively chemolithoautotrophic bacterium designated strain SRT was isolated from a terrestrial hot spring in Kamchatka (Russia). The cells of the novel strain were spore-forming rods with a Gram-positive type of cell wall. The novel isolate grew at 60–82 °C (optimum 75 °C) and pH 6.0–7.5 (optimum 6.8). Molecular hydrogen and formate were used as electron donors. Thiosulfate, sulfate or elemental sulfur served as electron acceptors yielding hydrogen sulfide. No growth was observed on either substrate in the presence of nitrate as the electron acceptor. The G+C content of the DNA was 56.2 mol%. Phylogenetic analyses of the 16S rRNA gene showed that strain SRT was most closely related to Ammonifex degensii (96.4 % gene sequence similarity). However, the novel isolate possessed phenotypic characteristics that differed significantly from those of A. degensii, the only other recognized species of the genus Ammonifex. It is concluded that strain SRT (=DSM 19636T=VKM B-2461T) represents the type strain of a novel species of the genus Ammonifex, for which the name Ammonifex thiophilus sp. nov. is proposed. An emendation of the genus Ammonifex is proposed based on the phenotypic characteristics of the novel species.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SRT is EF554597.






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