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Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake

¹ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
² Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba-ken 278-0043, Japan

Correspondence
M. C. Gutiérrez
mcg{at}us.es

A novel extremely halophilic strain, designated XH-65^T, isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65^T is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum at 3.4 M NaCl, and grows at pH 6.0–9.0, with optimum growth at pH 7.5. Strain XH-65^T grows at 25–50 °C, with optimal growth at 37 °C. Magnesium is not required for growth. On the basis of 16S rRNA gene sequence analysis, strain XH-65^T was shown to belong to the genus Haloterrigena and was related to Haloterrigena turkmenica VKM B-1734^T (98.1 % sequence similarity), Haloterrigena saccharevitans AB14^T (96.9 %), Haloterrigena thermotolerans PR5^T (96.3 %), Haloterrigena limicola AX-7^T (95.8 %) and Haloterrigena hispanica FP1^T (95.7 %). DNA–DNA hybridization revealed 37 % relatedness between strain XH-65^T and Htg. turkmenica VKM B-1734^T. The polar lipid composition revealed the presence of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (12)-glucose glycerol diether (S₂-DGD). The results of the DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain XH-65^T from the six Haloterrigena species with validly published names. Therefore, strain XH-65^T represents a novel species, for which the name Haloterrigena salina sp. nov. is proposed, with the type strain XH-65^T (=CGMCC 1.6203^T =JCM 13891^T).

A thin-layer chromatogram of polar lipids of strain XH-65^T and related haloarchaea is available as supplementary material with the online version of this paper.

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