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Int J Syst Evol Microbiol 58 (2008), 2871-2875; DOI  10.1099/ijs.0.2008/000703-0
© 2008 International Union of Microbiological Societies

Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba

Chang Fu Tian1, En Tao Wang1,2, Li Juan Wu1, Tian Xu Han1, Wen Feng Chen1, Chun Tao Gu1, Jin Gang Gu3 and Wen Xin Chen1

1 Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, 100094 Beijing, PR China
2 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico
3 Institute of Agricultural Resources and Regional Planning of Chinese Academy of Agricultural Sciences, 100081 Beijing, PR China

Correspondence
Wen Xin Chen
wxchencau{at}yahoo.com.cn

Six strains were isolated from root nodules of Vicia faba grown in Nanchang, Yifeng, Taihu, Huaibei, Bengbu and Lujiang, in the middle and lower reaches of the Yangtze River. According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species. CCBAU 33202T, a representative of these novel isolates, showed sequence similarity to its closest relatives R. etli CFN 42T and R. leguminosarum USDA 2370T of 99.5 and 99.1 % for the 16S rRNA gene, 91.9 and 91.9 % for atpD and 90.3 and 93.2 % for recA. The strains from this study could also be differentiated from R. etli CFN 42T and R. leguminosarum USDA 2370T by 16S–IGS RFLP and SDS-PAGE of whole-cell proteins, fatty acid profiles and several phenotypic characteristics. DNA–DNA hybridization yielded relatedness of 19 and 14–43 %, respectively, with R. etli CFN 42T and strains representing different biovars of R. leguminosarum. All data obtained in this study showed that these V. faba isolates belong to a novel species, for which the name Rhizobium fabae sp. nov. is proposed. The type strain, CCBAU 33202T (=LMG 23997T =JCM 14381T), was isolated from Nanchang.

The GenBank/EMBL/DDBJ accession numbers for the recA and atpD sequences of strains CCBAU 23122, CCBAU 23123, CCBAU 23127, CCBAU 23132, CCBAU 33201 and CCBAU 33202T are respectively EF579936–EF579941 (recA) and EF579924–EF579929 (atpD). Those for the nodD sequences of strains CCBAU 33202T and CCBAU 23123 are EU430078 and EU430079.

Neighbour-joining trees based on recA, atpD and nodD sequences and dendrograms based on 16S–IGS RFLP and whole-cell protein SDS-PAGE profiles are available as supplementary material with the online version of this paper.






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