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Halomonas daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield soil

¹ State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China
² Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, PR China
³ Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto City, Kumamoto 860-8555, Japan

Correspondence
Xiao-Lei Wu
xiaolei_wu{at}tsinghua.edu.cn
Ya-Nang Wang
wangyanan{at}mail.tsinghua.edu.cn

A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30^T, was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1–15 % (w/v) [optimum at 5–10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Halomonas in the class Gammaproteobacteria; the highest 16S rRNA gene sequence similarities were with Halomonas desiderata DSM 9502^T (98.8 %), Halomonas campisalis A4^T (96.6 %) and Halomonas gudaonensis CGMCC 1.6133^T (95.1 %). The major cellular fatty acids of strain DQD2-30^T were C_{18 : 1}7c (43.97 %), C_{19 : 0} cyclo 8c (23.37 %) and C_{16 : 0} (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA–DNA hybridization values of strain DQD2-30^T with the most closely related species of the genus Halomonas were 51.8 %, 28.4 % and 23.5 % for H. desiderata, H. campisalis and H. gudaonensis, respectively. Based on these analyses, strain DQD2-30^T (=CGMCC 1.6443^T=LMG 23896^T) is proposed to represent the type strain of a novel species, Halomonas daqingensis sp. nov.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DQD2-30^T is EF121854.

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