IJSEM Journal of Bacteriology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figure
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyazaki, M.
Right arrow Articles by Horikoshi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyazaki, M.
Right arrow Articles by Horikoshi, K.
Agricola
Right arrow Articles by Miyazaki, M.
Right arrow Articles by Horikoshi, K.
Int J Syst Evol Microbiol 58 (2008), 2815-2820; DOI  10.1099/ijs.0.65826-0
© 2008 International Union of Microbiological Societies

Amphritea japonica sp. nov. and Amphritea balenae sp. nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan

Masayuki Miyazaki1, Yuichi Nogi1, Yoshihiro Fujiwara1, Masaru Kawato1, Takahiko Nagahama1, Kaoru Kubokawa2 and Koki Horikoshi1

1 Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
2 Center for Advanced Marine Research, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan

Correspondence
Masayuki Miyazaki
miyazakim{at}jamstec.go.jp

Two novel species were isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of about 230 m. The isolated strains, JAMM 1866T, JAMM 1548 and JAMM 1525T, were Gram-negative, rod-shaped, non-spore-forming and motile by means of a single polar or bipolar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences of strains JAMM 1866T and JAMM 1548 indicated a relationship to the symbiotic bacterial clone R21 of Osedax japonicus (100 % sequence similarity) and all three isolates were closely related to Amphritea atlantica (97.7–97.8 % similarity) within the class Gammaproteobacteria. The novel isolates were able to produce isoprenoid quinone Q-8 as the major component. The predominant fatty acids were C16 : 0, C16 : 1 and C18 : 1, with C12 : 1 3-OH present in smaller amounts. The DNA G+C contents of the three isolated strains were about 47 mol%. Based on differences in taxonomic characteristics, the three isolated strains represent two novel species of the genus Amphritea for which the names Amphritea japonica sp. nov. (type strain JAMM 1866T=JCM 14782T=ATCC BAA-1530T; reference strain JAMM 1548) and Amphritea balenae sp. nov. (type strain JAMM 1525T=JCM 14781T=ATCC BAA-1529T) are proposed.

Abbreviations: ML, maximum likelihood; NJ, neighbour-joining

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains JAMM 1866T, JAMM 1548 and JAMM 1525T are AB330881–AB330883, respectively.

Transmission electron micrographs of negatively stained cells of the two novel species described in this study are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2008 by the International Union of Microbiological Societies.