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Klugiella xanthotipulae gen. nov., sp. nov., a novel member of the family Microbacteriaceae

University of Georgia, Department of Microbiology, Athens, GA 30602, USA

Correspondence
Joy Doran Peterson
jpeterso{at}uga.edu

An actinobacterium, designated strain 44C3^T, was isolated in Michigan, USA, from the hindgut of the larvae of Tipula abdominalis, an aquatic crane fly, and was subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a separate clade within the family Microbacteriaceae. It showed highest 16S rRNA gene sequence similarity with Cryobacterium psychrotolerans 0549^T (96.5 %). Strain 44C3^T had a novel B-type peptidoglycan. The peptidoglycan contained the diamino acid lysine, the peptide Gly–D-Glu was detected in the partial hydrolysate and alanine was the N terminus of the interpeptide bridge. No other amino acids found in other B-type peptidoglycans (including diaminobutyric acid, ornithine, homoserine and hydroxyglutamic acid) could be detected. The major menaquinones were MK-12 and MK-11, the major fatty acids were ai-C_{15 : 0}, ai-C_{17 : 0} and i-C_{16 : 0} and the DNA G+C content was 60.9 mol%. Analysis of the chemotaxonomic and phylogenetic data suggested that strain 44C3^T represented a novel species of a new genus within the family Microbacteriaceae, for which the name Klugiella xanthotipulae gen. nov., sp. nov. is proposed. The type strain of Klugiella xanthotipulae is 44C3^T (=DSM 18031^T =ATCC BAA-1524^T).

A minimum-evolution phylogenetic dendrogram based on 16S rRNA gene sequence similarity is available as supplementary material with the online version of this paper.

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