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Int J Syst Evol Microbiol 58 (2008), 2640-2645; DOI  10.1099/ijs.0.65776-0
© 2008 International Union of Microbiological Societies

Paenibacillus taichungensis sp. nov., from soil in Taiwan

Fwu-Ling Lee1, Chih-Jen Tien1, Chun-Ju Tai1, Li-Ting Wang1, Yung-Chuan Liu2 and Lih-Ling Chern1,{dagger}

1 Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30052, Taiwan, ROC
2 Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan, ROC

Correspondence
Fwu-Ling Lee
fll{at}firdi.org.tw

Among a large collection of Taiwanese soil isolates, a novel Gram-variable, rod-shaped, motile, endospore-forming bacterial strain, strain V10537T, was subjected to a polyphasic study including 16S rRNA and gyrB gene sequence analysis, DNA–DNA hybridization experiments, cell wall peptidoglycan type, cellular fatty acid composition analysis and comparative phenotypic characterization. 16S rRNA gene sequence analysis indicated that the organism belonged to the genus Paenibacillus. Strain V10537T possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. It contained menaquinone MK-7 as the predominant isoprenoid quinone and anteiso-C15 : 0 (53.6 %) and C16 : 0 (19.0 %) as the major fatty acids. Phylogenetically, the most closely related species to strain V10537T were Paenibacillus pabuli, Paenibacillus xylanilyticus, Paenibacillus amylolyticus, Paenibacillus barcinonensis and Paenibacillus illinoisensis, with 16S rRNA gene sequence similarities of 99.5, 98.8, 98.3, 98.2 and 98.1 % to the respective type strains. The gyrB gene sequence similarities between strain V10537T and these strains were 76.9–85.0 %. DNA–DNA hybridization experiments showed levels of relatedness of 8.5–45.6 % between strain V10537T and these strains. The DNA G+C content of strain V10537T was 46.7 mol%. Strain V10537T was clearly distinguishable from other Paenibacillus species and thus represents a novel species of the genus Paenibacillus, for which the name Paenibacillus taichungensis sp. nov. is proposed. The type strain is V10537T (=BCRC 17757T =DSM 19942T).

{dagger}Present address: Graduate Institute of Plant Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain V10537T is EU179327; accession numbers for gyrB gene sequences of the six strains used in this study are EU272024–EU272029.

Maximum-parsimony and maximum-likelihood trees of Paenibacillus strains based on 16S rRNA and gyrB gene sequences are available as supplementary material with the online version of this paper.






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