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Int J Syst Evol Microbiol 58 (2008), 2459-2464; DOI  10.1099/ijs.0.65655-0
© 2008 International Union of Microbiological Societies

Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil

Ivan D. Sorokin1, Irina K. Kravchenko1, Tatjana P. Tourova1, Tatjana V. Kolganova2, Eugenia S. Boulygina2 and Dimitry Yu. Sorokin1,3

1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia
2 Bioengineering Center, Russian Academy of Sciences, Moscow, Russia
3 Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

Correspondence
Dimitry Yu. Sorokin
soroc{at}inmi.host.ru
or
D.Y.Sorokin{at}tnw.tudelft.nl

Strain MS 6T was obtained from a microoxic enrichment with a soda soil sample from north-eastern Mongolia in nitrogen-free alkaline medium at pH 10. The isolate had clostridia-like motile cells and formed ellipsoid endospores. It was able to fix dinitrogen gas growing on nitrogen-free alkaline medium. Strain MS 6T was a strictly fermentative bacterium without a respiratory chain, although it had a high catalase activity and tolerated aerobic conditions. It was an obligate alkaliphile with a pH range for growth between 7.5 and 10.6 (optimum at 9.0–9.5). Growth and nitrogen fixation at pH 10 were possible at a total salt content of up to 1.2 M Na+ (optimum at 0.2–0.3 M). The dominant cellular fatty acids included C16 : 0, C16 : 1{omega}7, anteiso-C15 : 0 and C14 : 0. The dominant isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. 16S rRNA gene sequencing identified strain MS 6T as a member of the genus Bacillus. Its closest relative was Bacillus arseniciselenatis E1HT. The key functional nitrogenase gene nifH was detected in both strain MS 6T and its close relative and these strains formed a novel lineage in the nifH gene family. On the basis of these phenotypic and genetic comparisons, strain MS 6T is proposed to represent a novel species of the genus Bacillus, Bacillus alkalidiazotrophicus sp. nov. with the type strain MS 6T (=NCCB 100213T=UNIQEM U377T).

Abbreviations: NF, nitrogen fixation

Supplementary figures showing the cell morphology of strain MS 6T grown anaerobically at 0.6 M Na+ and pH 10 with glucose and two additional phylogenetic trees are available with the online version of this paper.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and nifH gene sequences for Bacillus alkalidiazotrophicus sp. nov. MS 6T are EU143680 and EU204959, respectively. The GenBank/EMBL/DDBJ accession number for the nifH gene sequence of Bacillus arseniciselenatis E1HT determined in this study is EU204960.






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