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Desulfovibrio marinisediminis sp. nov., a novel sulfate-reducing bacterium isolated from coastal marine sediment via enrichment with Casamino acids

¹ Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa 1-1, Hachioji 192-0397, Japan
² Research Institute of Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1 Tsukuba Central 6, Tsukuba 305-8566, Japan

Correspondence
Susumu Takii
takiisus{at}gmail.com

To obtain amino acid-utilizing sulfate reducers, enrichment culture was carried out with a medium containing Casamino acids and sulfate and inoculated with coastal marine sediment from the eutrophic Tokyo Bay, Japan. A sulfate reducer, designated strain C/L2^T, was isolated from the sulfide-producing enrichment culture after further enrichment with lactate and sulfate by means of the agar shake dilution method. Cells of strain C/L2^T were vibrio-shaped, Gram-negative, motile rods (0.7–1.0 µm wide and 1.0–3.5 µm long) with single polar flagella. The optimum temperature for its growth was 37 °C, the optimum pH was around 7.5 and the optimum NaCl concentration was 20–25 g l^–1. Hydrogen, formate, lactate, pyruvate, fumarate, malate, succinate, ethanol, propanol, glycerol, glycine, alanine, serine, aspartate, Casamino acids, peptone and yeast extract were used as electron donors. Sulfate, sulfite and thiosulfate each served as an electron acceptor, but elemental sulfur, nitrate, fumarate, acrylate and 2,4,6-tribromophenol did not. Disproportionation of thiosulfate was not observed. Desulfoviridin, c-type cytochromes and catalase were present. The major respiratory quinone was MK-6(H₂). The G+C content of the genomic DNA was 46.2 mol%. Comparisons based on 16S rRNA gene sequences and on dissimilatory sulfite reductase gene sequences clearly showed that strain C/L2^T belonged to the genus Desulfovibrio: its closest relatives were the uncharacterized Desulfovibrio sp. strain TBP-1 (16S rRNA gene sequence similarity of 99.4 %) and Desulfovibrio acrylicus DSM 10141^T (16S rRNA gene sequence similarity of 98.7 %). The level of DNA–DNA hybridization with Desulfovibrio acrylicus DSM 10141^T was 10.3 %. On the basis of the data from this study and the physiological and phylogenetic differences that exist between the isolate and Desulfovibrio acrylicus, strain C/L2^T represents a novel species of the genus Desulfovibrio, for which the name Desulfovibrio marinisediminis sp. nov. is proposed. The type strain is C/L2^T (=NMRC 101113^T=JCM 14577^T=DSM 17456^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and dsrAB gene sequences of strain C/L2^T are AB353727 and AB218445, respectively.

A phase-contrast microphotograph of cells of strain C/L2^T and a phylogenetic tree based on dsrAB gene sequences are available as supplementary material with the online version of this paper.