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Halomicrobium katesii sp. nov., an extremely halophilic archaeon

¹ Departamento de Microbiología, Facultad de Farmacia Campus de Cartuja s/n, 18071 Granada, Spain
² Institut de Nutrition de l'Alimentation et des Technologies Agro-Alimentaires, Université Mentouri, Constantine, Algeria
³ Departamento de Tecnología Medica, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Campus Coloso s/n, Antofagasta, Chile
⁴ Institut des Sciences de la Nature Faculté des Sciences, Université Mentouri, Constantine, Algeria
⁵ Laboratorio de Microbiología Ambiental, Universidad Católica de Valparaíso, Valparaíso, Chile

Correspondence
Mercedes Monteoliva-Sánchez
mmonteol{at}ugr.es

Two extremely halophilic archaea, strains Al-5^T and K-1, were isolated from Lake Tebenquiche (Atacama Saltern, Chile) and Ezzemoul sabkha (Algeria), respectively. Cells of the two strains were short-rod-shaped and Gram-negative; colonies were orange-pigmented. They grew optimally at 37–40 °C and pH 7.0–7.5 in the presence of 25 % (w/v) NaCl. Magnesium was not required. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester, the absence of phosphatidylglycerosulfate, and the presence of sulfated diglycosyl diether and diether diglycosyl as the sole glycolipids. DNA G+C contents of strains Al-5^T and K-1 were 52.4 and 52.9 mol% (T_m method), respectively. 16S rRNA gene sequence comparison with database sequences showed that strains Al-5^T and K-1 were most closely related to Halomicrobium mukohataei DSM 12286^T (similarities of 97.5 and 96.9 %, respectively). DNA–DNA hybridization indicated that strains Al-5^T and K-1 were members of a single species. However, DNA–DNA relatedness to Halomicrobium mukohataei was 55.7±2.5 %. A comparative analysis of phenotypic characteristics and DNA–DNA hybridization between the isolates and Halomicrobium mukohataei DSM 12286^T supported the conclusion that Al-5^T and K-1 represent a novel species within the genus Halomicrobium, for which the name Halomicrobium katesii sp. nov. is proposed. The type strain is Al-5^T (=CECT 7257^T=DSM 19301^T).

A phylogenetic tree, based on 16S rRNA gene sequences and constructed using the maximum-parsimony algorithm, of isolates Al-5^T and K-1 and related species is available with the online version of this paper.