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Sphingopyxis ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea

¹ Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
² Department of Biology and Medicinal Science, Pai Chai University, 14 Yeonja-1-Gil, Seo-Gu, Daejeon 302-735, Republic of Korea
³ Insect Resources Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea

Correspondence
Wan-Taek Im
wandra{at}kaist.ac.kr
Sung-Taik Lee
e_stlee{at}kaist.ac.kr

A Gram-negative, aerobic, motile, rod-shaped bacterium (strain Gsoil 250^T) was isolated from soil of a ginseng field in Pocheon province (South Korea) and was characterized using a polyphasic approach in order to determine its taxonomic position. Comparative analysis of 16S rRNA gene sequences showed that strain Gsoil 250^T belonged to the family Sphingomonadaceae of the class Alphaproteobacteria, and was related to Sphingopyxis macrogoltabida (98.7 %), Sphingopyxis chilensis (98.2 %), Sphingopyxis alaskensis (97.9 %), Sphingopyxis taejonensis (97.9 %) and Sphingopyxis witflariensis (97.8 %). The phylogenetic distance from any other species with validly published names within the genus Sphingopyxis was greater than 3.8 %. The G+C content of the genomic DNA of strain Gsoil 250^T was 69.2 mol%. Strain Gsoil 250^T contained Q-10 as the predominant respiratory lipoquinone and C_{18 : 1}7c and summed feature 4 (C_{16 : 1}7c and/or iso-C_{15 : 0} 2-OH; 34.6 %) as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids consisted of sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and two unknown glycolipids. Phenotypic and chemotaxonomic data supported the affiliation of strain Gsoil 250^T to the genus Sphingopyxis. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 250^T from the eight recognized Sphingopyxis species. Strain Gsoil 250^T therefore represents a novel species, for which the name Sphingopyxis ginsengisoli sp. nov. is proposed, with the type strain Gsoil 250^T (=KCTC 12582^T=LMG 23390^T).

Figures showing a transmission electron micrograph of a negatively stained single cell of strain Gsoil 250^T and a two-dimensional, thin-layer chromatograph of the polar lipids and a table detailing the cellular fatty acid profiles of strain Gsoil 250^T and related species of the genus Sphingopyxis are available as supplementary material with the online version of this paper.