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Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea

¹ Observatoire Océanologique de Banyuls, Laboratoire ARAGO, Université Pierre et Marie Curie - Paris 6, UMR 7621, F-75005 Paris, France
² Institut National des Sciences de l'Univers (INSU) CNRS, UMR 7621, BP 44, F-66650 Banyuls-sur-Mer, France
³ Laboratoire de Microbiologie, de Géochimie et d'Ecologie Marines, CNRS UMR 6117, Centre d'Océanologie de Marseille, Campus de Luminy, Case 901, 13288 Marseille Cedex 9, France
⁴ Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France

Correspondence
Philippe Lebaron
lebaron{at}obs-banyuls.fr

Two novel Gram-negative bacteria, designated strains DR41_21^T and DR41_18^T, were isolated from coastal, surface waters of the north-western Mediterranean Sea. The cells were motile, pleomorphic rods, 2.9 µm long and 0.9 µm wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 60 mol%. Phylogenetic analysis of 16S rRNA gene sequences positioned the isolates in the class Alphaproteobacteria within the family Rhodospirillaceae. The 16S rRNA gene sequence similarity of the two strains was 98.8 % but DNA–DNA hybridization indicated only 55 % relatedness. Strain DR41_21^T was able to denitrify and possessed nirK and nosZ genes, unlike strain DR41_18^T, which possessed only nirK. These isolates represent two novel species of a new genus, Nisaea gen. nov., for which the names Nisaea denitrificans sp. nov. and Nisaea nitritireducens sp. nov. are proposed. The type strain of Nisaea denitrificans is DR41_21^T (=DSM 18348^T=CIP 109265^T=OOB 129^T) and the type strain of Nisaea nitritireducens is DR41_18^T (=DSM 19540^T=CIP 109601^T=OOB 128^T).

An electron micrograph of a cell of strain DR41_21^T, the effects of pH, NaCl and temperature on the growth of the strain, the fatty acid and polar lipid compositions of strains DR41_18^T and DR41_21^T, neighbour-joining phylogenetic trees for their nirK and nosZ gene sequences and a graph of the growth of strain DR41_21^T under anaerobiosis with nitrate and nitrite or nitrate, nitrite and nitrous oxide as electron acceptors are available as supplementary material with the online version of this paper.