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Int J Syst Evol Microbiol 58 (2008), 2330-2335; DOI  10.1099/ijs.0.65515-0
© 2008 International Union of Microbiological Societies

Bacillus cecembensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas

G. S. N. Reddy, Anarasi Uttam and S. Shivaji

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

Correspondence
S Shivaji
shivas{at}ccmb.res.in

Strain PN5T is a Gram-positive, aerobic, motile, rod-shaped, peritrichously flagellated bacterium that was isolated from the Pindari glacier using nutrient agar medium. Cells of PN5T are catalase-positive and oxidase-negative and contain lysine, glutamic acid and alanine in the peptidoglycan (peptidoglycan type A4{alpha}). Further, the cells are characterized by the presence of iso-C15 : 0 and iso-C16 : 1 as the predominant fatty acids and MK-7 as the isoprenoid quinone. Based on the above characteristics, strain PN5T was assigned to the genus Bacillus. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PN5T clustered with the type strain of Bacillus silvestris with a sequence similarity of 97.2 %. DNA–DNA hybridization between PN5T and B. silvestris DSM 12223T resulted in a relatedness of only 15 %, clearly indicating that strain PN5T represents a novel species. Further, PN5T was different from B. silvestris with respect to various phenotypic and chemotaxonomic characteristics. Therefore, strain PN5T is identified as a representative of a novel species of the genus Bacillus, for which the name Bacillus cecembensis sp. nov. is proposed. Bacillus cecembensis is unique among psychrotolerant Bacillus species in containing L-Lys–D-Glu in the cell-wall peptidoglycan. The type strain is PN5T (=LMG 23935T =MTCC9127T =JCM 15113T).

Abbreviations: Dap, diaminopimelic acid

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PN5T is AM773821.

A transmission electron micrograph of a cell of strain PN5T and 16S rRNA gene sequence-based trees generated by UPGMA, minimum evolution and DNAPARS are available as supplementary material with the online version of this paper.






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