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Nocardioides koreensis sp. nov., Nocardioides bigeumensis sp. nov. and Nocardioides agariphilus sp. nov., isolated from soil from Bigeum Island, Korea

Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea

Correspondence
Chang-Jin Kim
changjin{at}kribb.re.kr

Three Gram-positive, coccoid to rod-shaped actinobacteria, designated strains MSL-09^T, MSL-19^T and MSL-28^T, were isolated from a soil sample collected from Bigeum Island in Korea, and were subjected to a polyphasic taxonomic analysis. All three isolates grew optimally at pH 7.5–9.0 and 28–30 °C. 16S rRNA gene analysis revealed that all three strains belonged to the genus Nocardioides, with strains MSL-09^T, MSL-19^T and MSL-28^T respectively showing the highest sequence similarity to Nocardioides aquaticus DSM 11439^T (96.25 %), Nocardioides aquiterrae GW-9^T (95.75 %) and Nocardioides terrigena DS-17^T (95.61 %). Chemotaxonomically, they contain LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H₄) as the predominant menaquinone and diphosphatidylglycerol, phosphatidylglycerol and some unknown lipids as the polar lipids found in the cell wall. iso-C_{16 : 0} is a major fatty acid. The G+C content of the DNA was respectively 69.9, 69.3 and 69.4 mol% for strains MSL-09^T, MSL-19^T and MSL-28^T. Based on morphological, physiological, biochemical and chemotaxonomic characters presented in this study, the three strains represent novel species of the genus Nocardioides. The names Nocardioides koreensis sp. nov. (type strain MSL-09^T =KCTC 19272^T =DSM 19266^T), Nocardioides bigeumensis sp. nov. (type strain MSL-19^T =KCTC 19290^T =DSM 19320^T) and Nocardioides agariphilus sp. nov. (type strain MSL-28^T =KCTC 19276^T =DSM 19323^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains MSL-09^T, MSL-19^T and MSL-28^T are respectively EF466115, EF466114 and EF466113.

Scanning electron micrographs of cells of the novel strains are available as supplementary material with the online version of this paper.

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				J.-H. Yoon, S.-J. Kang, S. Park, W. Kim, and T.-K. Oh Nocardioides caeni sp. nov., isolated from wastewater Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2794 - 2797. [Abstract] [Full Text] [PDF]