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Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China

¹ Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea
² Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea
³ Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea
⁴ Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China

Correspondence
Chang-Jin Kim
changjin{at}kribb.re.kr

Two Gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5–7 % (w/v) NaCl were isolated from a salt lake, Xin-Jiang Province, China. The strains, designated BH312^T and BH314, grew in the presence of 1–18 % (w/v) NaCl and at temperatures of 15–50 °C (optimum: 40 °C) and pH values of 5.5–10.0 (optimum: pH 7.5–8.0). The major cellular fatty acids were anteiso-C_{15 : 0}, iso-C_{15 : 0} and anteiso-C_{17 : 0}. The DNA G+C content was 38.8–39.0 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates formed a cluster with Gracilibacillus orientalis XH-63^T within the genus Gracilibacillus. The levels of 16S rRNA gene sequence similarity with closely related type strains were lower than 96.2 %. On the basis of phylogenetic, phenotypic and chemotaxonomic properties, the isolates represent a novel species of the genus Gracilibacillus for which the name Gracilibacillus lacisalsi sp. nov. is proposed. The type strain is BH312^T (=KCTC 13129^T=DSM 19029^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains BH312^T and BH314 are DQ664540 and DQ664541, respectively.

A transmission electron micrograph showing general morphology of negatively stained cells of strain BH312^T and thin-layer chromatograms of polar lipids from strain BH312^T and related strains are available as supplementary material with the online version of this paper.

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