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Int J Syst Evol Microbiol 58 (2008), 2258-2265; DOI  10.1099/ijs.0.65727-0
© 2008 International Union of Microbiological Societies

Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney

Hiroyuki Imachi1, Sanae Sakai1,2, Hisako Hirayama1, Satoshi Nakagawa1, Takuro Nunoura1, Ken Takai1 and Koki Horikoshi1

1 Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2 Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan

Correspondence
Hiroyuki Imachi
imachi{at}jamstec.go.jp

A novel thermophilic, anaerobic bacterium, strain RASENT, was isolated from a deep-sea hydrothermal vent chimney sample collected in the Iheya North field, Okinawa Trough, Japan, at a water depth of 982 m. The cells were motile, Gram-negative and helical with hooked ends, 0.23–0.28x15–27 µm in size with an approximate wavelength of 1.1–1.5 µm. Growth of the strain was observed at 37–60 °C (optimum 50 °C), in 2.5–3.5 % (w/v) NaCl (optimum 2.5–3 % NaCl) and at pH 6.0–7.5 (optimum pH 7.0). The strain grew on yeast extract only of the substrates examined in this study. The G+C content of the genomic DNA was 27.1 mol%. Major fatty acids for the strain were C16 : 0, C18 : 1({Delta}9) trans, C18 : 0 and C18 : 1({Delta}9) cis. Based on comparative 16S rRNA gene sequence analysis, strain RASENT formed a deeply branching lineage within the phylum Spirochaetes and had only low levels of sequence similarity with other species of the phylum (range of similarity 72.1–80.6 %). Hence, we propose the name Exilispira thermophila gen. nov., sp. nov. The type strain of Exilispira thermophila is strain RASENT (=JCM 14728T =NBRC 103205T =KCTC 5595T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RASENT is AB364473.






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