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Leptospira weilii serovar Topaz, a new member of the Tarassovi serogroup isolated from a bovine source in Queensland, Australia

B. G. Corney^1,, A. T. Slack^2,, M. L. Symonds², M. F. Dohnt², C. S. McClintock³, M. R. McGowan³ and L. D. Smythe²

¹ Animal Research Institute, Queensland Department of Primary Industries and Fisheries, Yeerongpilly, Australia
² WHO/FAO/OIE Collaborating Centre for Reference & Research on Leptospirosis, Western Pacific Region, Communicable Diseases, Queensland Health Forensic and Scientific Services, Brisbane, Australia
³ University of Queensland, St Lucia, Australia

Correspondence
L. D. Smythe
Lee_Smythe{at}health.qld.gov.au

This paper reports on a Leptospira isolate of bovine origin and its identification as belonging to a previously unknown serovar, for which the name Topaz is proposed. The isolate (94-79970/3) was cultured from bovine urine from a north Queensland dairy farm in Australia. Strain 94-79970/3 grew at 30 °C in Ellinghausen McCullough Johnson Harris (EMJH) medium but failed to grow at 13 °C in EMJH medium or in the presence of 8-azaguanine. Serologically, strain 94-79970/3 produced titres against the Leptospira borgpetersenii serovar Tarassovi, the reference strain for the Tarassovi serogroup; however, no significant titres to any other serovars within the serogroup were obtained. Using 16S rRNA and DNA gyrase subunit B gene analysis, strain 94-79970/3 was identified as a member of the species Leptospira weilii. We propose that the serovar be named Topaz, after the location where the original isolate was obtained. The reference strain for this serovar is 94-79970/3 (=KIT 94-79970/3=LT722).

These authors contributed equally to this work and wish to be recognized as joint first authors.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrB gene sequences of Leptospira weilii serovar Topaz strain 94-79970/3 are DQ483058 and DQ483059, respectively.