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Int J Syst Evol Microbiol 58 (2008), 61-67; DOI  10.1099/ijs.0.65182-0
© 2008 International Union of Microbiological Societies

Clostridiisalibacter paucivorans gen. nov., sp. nov., a novel moderately halophilic bacterium isolated from olive mill wastewater

Pierre-Pol Liebgott1, Manon Joseph1, Marie-Laure Fardeau1, Jean-Luc Cayol1, Enevold Falsen2, Fatima Chamkh3, Abdel-Illah Qatibi3 and Marc Labat1

1 Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, F-13288 Marseille Cedex 9, France
2 CCUG, Culture Collection, University of Göteborg, Guldhedsgatan 10, SE-413 46 Göteborg, Sweden
3 Laboratoire de Génie Microbiologique, B.P. 549, Faculté des Sciences et Techniques, Université Cadi Ayyad, 40000 Marrakech, Morocco

Correspondence
Marc Labat
marc.labat{at}esil.univmed.fr

A moderately halophilic, strictly anaerobic bacterium, designated 37HS60T, was isolated from an olive mill wastewater in southern Morocco (Marrakesh). The cells were straight, motile and stained Gram-positive, forming spherical and terminal spores and with an atypical thick and stratified multilayered cell wall. Major fatty acid components were iso-C17 : 1{omega}10c or anteiso-C17 : 1{omega}3c (19.3 %), C14 : 0 (14.3 %), C16 : 1{omega}7c (9.9 %), C16 : 1{omega}7c DMA (8.5 %) and C16 : 0 (7.6 %). Strain 37HS60T grew from 20 to 50 °C with an optimum at 40–45 °C, and growth was observed from pH 5.5 to 8.5 with an optimum of 6.8. The salinity range for growth was 10–100 g NaCl l–1 with an optimum at 50 g NaCl l–1. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 37HS60T fell within the evolutionary radiation enclosed by cluster XII of the Clostridium subphylum. Strain 37HS60T exhibited highest 16S rRNA gene sequence similarity of 92.0 % with Caloranaerobacter azorensis and 90.6 % with Clostridium purinilyticum. Moreover, 37HS60T did not grow on basal medium with hexose or pentose sugars as carbon and energy sources. Pyruvate, fumarate and succinate were the best substrates for 37HS60T growth with 1.0 g yeast extract l–1. The DNA G+C content was 33.0 mol%. Due to its phenotypic and genotypic characteristics, isolate 37HS60T is proposed as a novel species of a new genus, Clostridiisalibacter paucivorans gen. nov., sp. nov. The type strain is 37HS60T (=JCM 14354T=CCUG 53849T).

Abbreviations: OMW, olive mill wastewater

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of 37HS60T is EF026082.






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