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Int J Syst Evol Microbiol 58 (2008), 40-44; DOI  10.1099/ijs.0.65307-0
© 2008 International Union of Microbiological Societies

Halomonas sabkhae sp. nov., a moderately halophilic bacterium isolated from an Algerian sabkha

K. Kharroub1,2, M. L. Jiménez-Pranteda1, M. Aguilera1, A. Boulahrouf3, A. Ramos-Cormenzana1 and M. Monteoliva-Sánchez1

1 Departamento de Microbiología, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain
2 Institut de Nutrition de l'Alimentation et des Technologies Agro-Alimentaires, Faculté des Sciences de la Nature et de la Vie, Université Mentouri, Constantine, Algeria
3 Département des Sciences de la Nature et de la Vie, Faculté des Sciences de la Nature et de la Vie, Université Mentouri, Constantine, Algeria

Correspondence
Mercedes Monteoliva-Sánchez
mmonteol{at}ugr.es

A novel moderately halophilic bacterium belonging to the genus Halomonas was isolated from brine samples collected from Ezzemoul sabkha in north-eastern Algeria. The cells of strain 5-3T were Gram-negative, rod-shaped and non-motile. The strain was catalase- and oxidase-positive and produced an exopolysaccharide. Growth occurred at NaCl concentrations of 5–25 % (optimum at 7.5 %), at 30–50 °C (optimum at 37–40 °C) and at pH 6.0–9.0 (optimum at pH 7.5). The major fatty acids were C12 : 0 3-OH, C16 : 1{omega}7c/iso-C15 : 0 2-OH, C16 : 0, C18 : 1{omega}7c and C19 : 0{omega}8c cyclo. The G+C content of the genomic DNA was 57.0 mol% (Tm). The affiliation of strain 5-3T with the genus Halomonas was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was Halomonas halmophila, which showed a 16S rRNA gene sequence similarity of 99.7 %. However, the level of DNA–DNA relatedness between the novel isolate and the related Halomonas species was less than 31.4 %. On the basis of the data from this polyphasic study, strain 5-3T represents a novel species of the genus Halomonas, for which the name Halomonas sabkhae sp. nov. is proposed. The type strain is 5-3T (=CECT 7246T=DSM 19122T=LMG 24084T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 5-3T is EF442769.

A maximum-parsimony phylogenetic tree, based on 16S rRNA gene sequences, for strain 5-3T, species of the genus Halomonas and other taxa comprising Gram-negative, halophilic bacteria is available with the online version of this paper.






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