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Int J Syst Evol Microbiol 58 (2008), 286-289; DOI  10.1099/ijs.0.65315-0
© 2008 International Union of Microbiological Societies

Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil

Hiroki Miwa1,2, Iftikhar Ahmed1,3, Jaewoo Yoon4, Akira Yokota4 and Toru Fujiwara1,2

1 Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan
2 SORST, JST, Chiyoda-Ku, Tokyo, Japan
3 National Agricultural Research Center, Park Road, Islamabad 45500, Pakistan
4 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Toru Fujiwara
atorufu{at}mail.ecc.u-tokyo.ac.jp
Hiroki Miwa
umiwa{at}mail.ecc.u-tokyo.ac.jp

A non-spore-forming, Gram-negative, motile, rod-shaped, boron-accumulating bacterium isolated from soil was characterized by polyphasic taxonomy. The strain, designated BAM-48T, was positive for catalase and oxidase activities and grew at 4–37 °C, 0–1 % NaCl and pH 5–9. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate BAM-48T was closely related to Variovorax paradoxus IAM 12373T (99.3 % sequence similarity), Variovorax soli GH9-3T (98.3 %) and Variovorax dokdonensis DS-43T (97.0 %). DNA–DNA hybridization values between strain BAM-48T and V. paradoxus NBRC 15149T, V. soli KACC 11579T and V. dokdonensis KCTC 12544T were only 49.1, 25.4 and 24.6 %, respectively. The major respiratory quinone was ubiquinone 8 (Q-8). The DNA G+C content was 71.2 mol%. Strain BAM-48T contained C16 : 0 (36.1 %), C16 : 1{omega}7c (21.4 %) and C17 : 0 cyclo (19.0 %) as the major fatty acids. Based on phenotypic, chemotaxonomic and phylogenetic data, strain BAM-48T should be classified as a member of a novel species in the genus Variovorax, for which the name Variovorax boronicumulans sp. nov. is proposed. The type strain is BAM-48T (=NBRC 103145T =KCTC 22010T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BAM-48T is AB300597.

Details of boron uptake by strain BAM-48T and related type strains are available as supplementary material with the online version of this paper.






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