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Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil

¹ Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan
² SORST, JST, Chiyoda-Ku, Tokyo, Japan
³ National Agricultural Research Center, Park Road, Islamabad 45500, Pakistan
⁴ Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Toru Fujiwara
atorufu{at}mail.ecc.u-tokyo.ac.jp
Hiroki Miwa
umiwa{at}mail.ecc.u-tokyo.ac.jp

A non-spore-forming, Gram-negative, motile, rod-shaped, boron-accumulating bacterium isolated from soil was characterized by polyphasic taxonomy. The strain, designated BAM-48^T, was positive for catalase and oxidase activities and grew at 4–37 °C, 0–1 % NaCl and pH 5–9. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate BAM-48^T was closely related to Variovorax paradoxus IAM 12373^T (99.3 % sequence similarity), Variovorax soli GH9-3^T (98.3 %) and Variovorax dokdonensis DS-43^T (97.0 %). DNA–DNA hybridization values between strain BAM-48^T and V. paradoxus NBRC 15149^T, V. soli KACC 11579^T and V. dokdonensis KCTC 12544^T were only 49.1, 25.4 and 24.6 %, respectively. The major respiratory quinone was ubiquinone 8 (Q-8). The DNA G+C content was 71.2 mol%. Strain BAM-48^T contained C_{16 : 0} (36.1 %), C_{16 : 1}7c (21.4 %) and C_{17 : 0} cyclo (19.0 %) as the major fatty acids. Based on phenotypic, chemotaxonomic and phylogenetic data, strain BAM-48^T should be classified as a member of a novel species in the genus Variovorax, for which the name Variovorax boronicumulans sp. nov. is proposed. The type strain is BAM-48^T (=NBRC 103145^T =KCTC 22010^T).

Details of boron uptake by strain BAM-48^T and related type strains are available as supplementary material with the online version of this paper.

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