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1 Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea
2 Educational Science Research Institute, Cheju National University, Jeju 690-756, Republic of Korea
3 Faculty of Biotechnology, Cheju National University, Jeju 690-756, Republic of Korea
Correspondence
Soon Dong Lee
sdlee{at}cheju.ac.kr
Two novel actinomycete strains, designated HFW-18 and HFW-21T, were isolated from a water sample around Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells of the organisms were aerobic, Gram-positive, non-motile rods. The temperature and initial pH ranges for growth were 4–37 °C and pH 5.1–9.1, respectively. They had LL-diaminopimelic acid in their cell-wall peptidoglycan, MK-8(H4) as the major menaquinone and DNA G+C contents of 71.1–72.2 mol%. The cellular fatty acids consisted of straight-chain saturated, branched, monounsaturated and 10-methyl fatty acids, with a major component of iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The isolates were identical in terms of their 16S rRNA gene sequences and BOX-PCR DNA fingerprints. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates occupied distinct positions within the radiation of the genus Nocardioides. According to 16S rRNA gene sequence similarity, the closest relatives were Nocardioides ganghwensis JC2055T (97.4 %), Nocardioides oleivorans DSM 16090T (97.3 %) and Nocardioides furvisabuli SBS-26T (97.0 %). Levels of 16S rRNA gene sequence similarity between the isolates and other members of the genus Nocardioides were in the range 91.8–95.3 %. On the basis of the phenotypic and molecular genetic data presented here, the isolates represent members of a novel species of the genus Nocardioides. The name Nocardioides hwasunensis sp. nov. is proposed, and the type strain is HFW-21T (=KCTC 19197T=DSM 18584T).
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences of strains HFW-18 and HFW-21T are AM295257 and AM295258, respectively.
A transmission electron micrograph of a cell of strain HFW-21T and a neighbour-joining tree of representatives of the family Nocardioidaceae, and a supplementary table of cellular fatty acid composition are available with the online version of this paper.
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