IJSEM Visit JGV Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figures
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hwang, C. Y.
Right arrow Articles by Cho, B. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hwang, C. Y.
Right arrow Articles by Cho, B. C.
Agricola
Right arrow Articles by Hwang, C. Y.
Right arrow Articles by Cho, B. C.
Int J Syst Evol Microbiol 58 (2008), 267-277; DOI  10.1099/ijs.0.65016-0
© 2008 International Union of Microbiological Societies

Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov.

Chung Yeon Hwang and Byung Cheol Cho

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea

Correspondence
Byung Cheol Cho
bccho{at}snu.ac.kr

Two novel Gram-negative bacterial strains, designated CL-GR15T and CL-GR35, were isolated from coastal seawater of the east coast of Korea. Identical 16S rRNA gene sequences were found in the two strains, and it was found that the strains represented a distinct and deep evolutionary lineage of descent in the order Rhizobiales, and clustered with yet-uncultured marine bacteria. This lineage could not be associated with any of 12 known families in the order Rhizobiales. The most closely related established genus was Ochrobactrum (90.7–92.5 % sequence similarity) in the family Brucellaceae. Cells of the strains were facultatively anaerobic, weakly motile rods which occasionally exhibited cohesion with each other. The strains were catalase-, oxidase- and gelatinase-positive, and accumulated intracellular poly-β-hydroxybutyrate granules. Ubiquinone 10 was the major quinone. The major polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminolipid and an unidentified glycolipid. The cellular fatty acids mainly consisted of C18 : 1{omega}7c, summed feature 3 (C15 : 0 iso 2-OH and/or C16 : 1{omega}7c) and C20 : 1{omega}7c. The DNA G+C content was 52.8–53.0 mol%. DNA–DNA hybridization experiments revealed high values (>97 %) for relatedness between strains CL-GR15T and CL-GR35 and suggested that these two strains constituted a single species. The distinct phylogenetic position and combinations of genotypic, phenotypic and chemotaxonomic characteristics support the proposal of Cohaesibacter gen. nov., with the type species Cohaesibacter gelatinilyticus sp. nov. (type strain, CL-GR15T=KCCM 42319T=DSM 18289T). Cohaesibacteraceae fam. nov. is also proposed.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CL-GR15T (=KCCM 42319T=DSM 18289T) is DQ386307.

Supplementary figures are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2008 by the International Union of Microbiological Societies.