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Int J Syst Evol Microbiol 58 (2008), 242-250; DOI  10.1099/ijs.0.65379-0
© 2008 International Union of Microbiological Societies

Physiological and phylogenetic characterization of a novel lithoautotrophic nitrite-oxidizing bacterium, ‘Candidatus Nitrospira bockiana’

E. V. Lebedeva1, M. Alawi2, F. Maixner3, P.-G. Jozsa2, H. Daims3 and E. Spieck2

1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Oktyabrya 7/2, Moscow 117312, Russia
2 Universität Hamburg, Biozentrum Klein Flottbek, Mikrobiologie, Ohnhorststr. 18, D-22609 Hamburg, Germany
3 Department für Mikrobielle Ökologie, Universität Wien, Althanstr. 14, A-1090 Wien, Austria

Correspondence
E. Spieck
spieck{at}mikrobiologie.uni-hamburg.de

A new isolate of a lithoautotrophic nitrite-oxidizing bacterium was obtained from internal corrosion deposits from a steel pipeline of the Moscow heating system. The organism oxidized nitrite as the sole energy source and fixed carbon dioxide as the only carbon source. The cells were extremely pleomorphic: loosely wound spirals, slightly curved and even straight rods were detected, as well as coccoid cells. The highest rate of nitrite consumption (1.5 mM nitrite as substrate) was measured at 42 °C, with a temperature range of 28–44 °C. In enrichment cultures with Nocardioides sp. as an accompanying organism, optimal oxidation of 5.8 mM nitrite occurred at 45 °C, with a range of 28–48 °C. Neither pyruvate nor yeast extract stimulated nitrification. Organotrophic growth was not observed. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel isolate represents a new sublineage of the genus Nitrospira. On the basis of physiological, chemotaxonomic and molecular characteristics, the name ‘Candidatus Nitrospira bockiana’ is proposed.

Abbreviations: AOB, ammonia-oxidizing bacteria; DGGE, denaturing gradient gel electrophoresis; EPS, extracellular polymeric substances; FISH, fluorescence in situ hybridization; ICMs, intracytoplasmic membranes; NB, nitrifying bacteria; NOB, nitrite-oxidizing bacteria

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of ‘Candidatus Nitrospira bockiana’ and the enrichment clone are EU084879 and EU084880, respectively.

A supplementary figure of the DGGE profile showing the increasing purity of the enrichments is available with the online version of this paper.




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 Proc. Natl. Acad. Sci. USAHome page
R. Hatzenpichler, E. V. Lebedeva, E. Spieck, K. Stoecker, A. Richter, H. Daims, and M. Wagner
A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring
PNAS, February 12, 2008; 105(6): 2134 - 2139.
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