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Enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment

¹ Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland
² BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, 9000 Gent, Belgium
³ Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, Lausanne, Switzerland

Correspondence
Roger Stephan
stephanr{at}fsafety.uzh.ch

Six Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (n=3), infant formula (n=2) and an infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated the isolates to the family Enterobacteriaceae. The highest rpoB gene sequence similarities (91.2–95.8 %) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species was supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by their ability to utilize melibiose, sucrose, D-arabitol, mucate and 1-O-methyl--galactopyranoside and their negative reactions for D-sorbitol utilization and the Voges–Proskauer test. On the basis of the phylogenetic analyses, DNA–DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel species of the genus Enterobacter, Enterobacter pulveris sp. nov. The type strain is 601/05^T (=LMG 24057^T=DSM 19144^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 1160/04, 516/05, 601/05^T, E441, E443 and E444 are DQ273686, DQ273682, DQ273684, EF472537, EF614996 and EF059835, respectively. The accession numbers for the rpoB gene sequences of strains 1160/04, 516/05, 601/05^T, E441, E443 and E444 are EF486514, EF486513, EF486512, EF632062, EF632063 and EF632064, respectively.

A neighbour-joining tree of 16S rRNA gene sequences showing the estimated phylogenetic relationships between Enterobacter pulveris sp. nov. and related genera within the family Enterobacteriaceae is available with the online version of this paper.

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