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Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond

¹ Centro de Neurociências e Biologia Celular, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal
² Department of Biochemistry, University of Coimbra, 3001-401 Coimbra, Portugal

Correspondence
Milton S. da Costa
milton{at}ci.uc.pt

A low-G+C, Gram-positive isolate, designated strain CVS-8^T, was isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago. This organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35–37 °C and an optimum pH between 7.5 and 8.0. Optimal growth occurred in media containing 4–6 % (w/v) NaCl and no growth occurred in medium without NaCl. The cell-wall peptidoglycan was of the A1 type with meso-diaminopimelic acid, the major respiratory quinone was MK-7, the major fatty acids were iso-15 : 0, 16 : 0, anteiso-15 : 0 and iso-16 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminoglycophospholipid. The G+C content of the DNA was 37.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-8^T represented a novel species of the genus Bacillus, the highest levels of sequence similarity (mean pairwise similarity values of 97.5 %) being found with respect to the type strains of Bacillus shackletonii and Bacillus acidicola. On the basis of the phylogenetic, physiological and biochemical data, strain CVS-8^T represents a novel species of the genus Bacillus, for which the name Bacillus isabeliae sp. nov. is proposed. The type strain is CVS-8^T (=LMG 22838^T=CIP 108578^T).

A maximum-parsimony phylogenetic dendrogram based on 16S rRNA gene sequences and a graph showing the effect of salt on growth of strain CVS-8^T are available as supplementary figures with the online version of this paper.