HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures Erratum Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Guo, Y. Articles by Huang, Y. Search for Related Content PubMed PubMed Citation Articles by Guo, Y. Articles by Huang, Y. Agricola Articles by Guo, Y. Articles by Huang, Y.

A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
² Department of Biology, Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China

Correspondence
Ying Huang
huangy{at}im.ac.cn

Streptomycetes are a complex group of actinomycetes that produce diverse bioactive metabolites of commercial significance. Systematics can provide a useful framework for identifying species that may produce novel metabolites. However, previously proposed approaches to the systematics of Streptomyces have suffered from either poor interlaboratory comparability or insufficient resolution. In particular, the Streptomyces griseus 16S rRNA gene clade is the most challenging and least defined group within the genus Streptomyces in terms of phylogeny. Here we report the results of a multilocus sequence analysis scheme developed to address the phylogeny of this clade. Sequence fragments of six housekeeping genes, atpD, gyrB, recA, rpoB, trpB and 16S rRNA, were obtained for 53 reference strains that represent 45 valid species and subspecies. Analysis of each individual locus confirmed the suitability of loci and the congruence of single-gene trees for concatenation. Concatenated trees of three, four, five and all six genes were constructed, and the stability of the topology and discriminatory power of each tree were analysed. It can be concluded from the results that phylogenetic analysis based on multilocus sequences is more accurate and robust for species delineation within Streptomyces. A multilocus phylogeny of six genes proved to be optimal for elucidating the interspecies relationships within the S. griseus 16S rRNA gene clade. Our multilocus sequence analysis scheme provides a valuable tool that can be applied to other Streptomyces clades for refining the systematic framework of this genus.

The GenBank/EMBL/DDBJ accession numbers for the sequence data reported in this paper are listed in Table 1.

A series of supplementary figures showing the chromosomal locations of the genes used, and phylogenetic trees for each protein-coding locus and for concatenated multiple genes are available with the online version of this paper.

This article has been cited by other articles:

				L. S. Haggerty, F. J. Martin, D. A. Fitzpatrick, and J. O. McInerney Gene and genome trees conflict at many levels Phil Trans R Soc B, August 12, 2009; 364(1527): 2209 - 2219. [Abstract] [Full Text] [PDF]