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Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge

¹ Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
² Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
³ Department of Civil Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

Correspondence
Hiroyuki Imachi
imachi{at}jamstec.go.jp

A mesophilic, syntrophic, fatty-acid-oxidizing anaerobic strain, designated MPA^T, was isolated from granular sludge in a mesophilic upflow anaerobic sludge blanket reactor used to treat palm oil mill effluent. Cells were slightly curved, non-motile rods. Spore formation was not observed. The optimal temperature for growth was around 37 °C and optimal pH for growth was 7.0. Strain MPA^T was able to grow on crotonate or pentenoate plus butyrate in pure culture. In co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei, strain MPA^T was able to oxidize straight-chain saturated fatty acids with carbon chain lengths of C4–C18. The strain was unable to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, iron(III) or DMSO as an electron acceptor. The G+C content of the DNA was 45.0 mol%. Based on comparative 16S rRNA gene sequence analysis, strain MPA^T was found to be a member of the genus Syntrophomonas and was most closely related to the type strains of Syntrophomonas curvata and Syntrophomonas sapovorans (sequence similarities of 94 %). Genetic and phenotypic characteristics demonstrated that strain MPA^T represents a novel species, for which the name Syntrophomonas palmitatica sp. nov. is proposed. The type strain is MPA^T (=JCM 14374^T=NBRC 102128^T=DSM 18709^T).

The GenBank/EMBL/DDBJ accession numbers for the rrnA and rrnB operons of the 16S rRNA gene sequence of strain MPA^T are AB274039 and AB274040, respectively.

A phase-contrast micrograph showing the cell morphology of strain MPA^T is available as supplementary material with the online version of this paper.