IJSEM Visit JGV Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figures
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindner, A. S.
Right arrow Articles by Hodson, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindner, A. S.
Right arrow Articles by Hodson, D. J.
Agricola
Right arrow Articles by Lindner, A. S.
Right arrow Articles by Hodson, D. J.
Int J Syst Evol Microbiol 57 (2007), 1891-1900; DOI  10.1099/ijs.0.64541-0
© 2007 International Union of Microbiological Societies

Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer

Angela S. Lindner1, Adriana Pacheco1, Henry C. Aldrich2,{dagger}, Andria Costello Staniec3, Ilker Uz4,{ddagger} and David J. Hodson1,§

1 Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL 32611-6450, USA
2 Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, USA
3 Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244-1240, USA
4 Department of Soil and Water Sciences, University of Florida, Gainesville, FL 32611-0290, USA

Correspondence
Angela S. Lindner
alind{at}eng.ufl.edu

Strain CSC1T, a Gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago. Based on 16S rRNA gene sequence similarity, this strain was identified as a member of the Alphaproteobacteria, most closely related to an uncultured member of the Methylocystaceae as well as two cultured organisms, Methylocystis sp. L32 and Methylocystis sp. SC2. This strain differed from extant species in cell shape, size, expression of soluble methane monooxygenase and its unique spiny surface layers, composed of polysaccharide. DNA–DNA hybridization results showed only 3.8 % relatedness with Methylocystis echinoides NCIMB 13100 and 41.1 % relatedness with Methylocystis rosea SV97T. Based on these genotypic and physiological differences, this isolate is proposed as a member of a novel species of the genus Methylocystis, Methylocystis hirsuta sp. nov. (type strain CSC1T =ATCC BAA-1344T =DSM 18500T).

Abbreviations: PHB, poly-beta-hydroxybutyrate; pMMO, particulate methane mononoxygenase; sMMO, soluble methane monooxygenase; TEM, transmission electron microscopy

{dagger}Deceased 9 August 2005.

{ddagger}Present address: Gaziosmanpasa University, Tokat Meslek Yuksekokulu, Oda No: 26, Tasliciftlik kampusu, 60250 Tokat, Turkey.

§Present address: Staff Engineer, CH2M Hill, 155 Grand Avenue, Suite 1000, Oakland, CA 94612, USA.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial mxaF, mmoX and pmoA gene sequences of strain CSC1T are respectively DQ364433, DQ664499, DQ664498 and DQ364434.

Neighbour-joining trees based on partial mmoX and mxaF gene sequences and results of electron microscope cytochemistry of the S-layer of strain CSC1T are available as supplementary material with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2007 by the International Union of Microbiological Societies.