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Int J Syst Evol Microbiol 57 (2007), 1846-1850; DOI  10.1099/ijs.0.64685-0
© 2007 International Union of Microbiological Societies

Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA–DNA hybridization in the Bacillus subtilis group

Li-Ting Wang1, Fwu-Ling Lee1, Chun-Ju Tai1 and Hiroaki Kasai2

1 Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30099, Taiwan
2 Marine Biotechnology Institute, Heita, Kamaishi, Iwate, 026-0001, Japan

Correspondence
Fwu-Ling Lee
fll{at}firdi.org.tw

The Bacillus subtilis group comprises eight closely related species that are indistinguishable from one another by 16S rRNA gene sequence analysis. Therefore, the gyrB gene, which encodes the subunit B protein of DNA gyrase, was selected as an alternative phylogenetic marker. To determine whether gyrB gene sequence analysis could be used for phylogenetic analysis and species identification of members of the B. subtilis group, the congruence of gyrB grouping with both 16S rRNA gene sequencing and DNA–DNA hybridization data was evaluated. Ranges of gyrB nucleotide and translated amino acid sequence similarities among the eight type strains were 75.4–95.0 % and 88.5–99.2 %, respectively, whereas 16S rRNA gene sequence similarities were 98.1–99.8 %. Results showed that gyrB gene sequences provide higher resolution than 16S rRNA gene sequences. The classification achieved by gyrB sequence analysis was in agreement with results obtained with DNA–DNA hybridization. It is concluded that the gyrB gene may be an efficient alternative target for identification and taxonomic analysis of members of the B. subtilis group.

The GenBank/EMBL/DDBJ accession numbers for the gyrB gene sequences of 32 strains used in this study are DQ309293–DQ309325 and those of the 16S rRNA gene sequences are DQ993674–DQ993679, EF423592–EF423609 and EF433402–EF433411, as detailed in Supplementary Table S1.

Details of strains and sequence accession numbers and a table of DNA–DNA reassociation values and gyrB and 16S rRNA gene sequences similarities are available as supplementary material with the online version of this paper.




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