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Alkalimonas collagenimarina sp. nov., a psychrotolerant, obligate alkaliphile isolated from deep-sea sediment

Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan

Correspondence
Atsushi Kurata
akurata{at}jamstec.go.jp

A psychrotolerant, obligately alkaliphilic, collagenolytic enzyme-producing bacterium, strain AC40^T, was isolated from a deep-sea sediment off Torishima Island, Japan, at a depth of 4026 m. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus Alkalimonas, with highest sequence similarity (97.9 %) to Alkalimonas delamerensis 1E1^T. DNA–DNA hybridization experiments of strain AC40^T with A. delamerensis 1E1^T revealed a level of relatedness of less than 30 %. Cells of strain AC40^T were strictly aerobic, rod-shaped, Gram-negative and motile by means of a single polar flagellum. The organism grew over a range of temperatures from 5 to 37 °C and at initial pH values between 7.0 and 10.5. Optimal growth was observed at 33 °C and at pH 8.5–10.0. Cellular fatty acids of strain AC40^T were predominantly saturated and mono-unsaturated straight-chain components (C_{16 : 0} and C_{18 : 1}). The major isoprenoid quinone was Q-8. The G+C content of the DNA was 49.3 mol%. Phylogenetic characteristics, physiological properties and DNA–DNA hybridization data indicate that strain AC40^T represents a novel species of the genus Alkalimonas, for which the name Alkalimonas collagenimarina sp. nov. is proposed. The type strain is AC40^T (=JCM 14267^T=NCIMB 14266^T).

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