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Int J Syst Evol Microbiol 57 (2007), 1521-1526; DOI  10.1099/ijs.0.64965-0
© 2007 International Union of Microbiological Societies

Description of Azospira restricta sp. nov., a nitrogen-fixing bacterium isolated from groundwater

Hee-Sung Bae1, Brian A. Rash1, Fred A. Rainey1, M. Fernanda Nobre2, Igor Tiago2, Milton S. da Costa3 and William M. Moe4

1 Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
2 Departamento de Zoologia and Centro de Neurociências, Universidade de Coimbra, 3004-517 Coimbra, Portugal
3 Departamento de Bioquímica and Centro de Neurociências, Universidade de Coimbra, 3001-401 Coimbra, Portugal
4 Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA

Correspondence
William M. Moe
moemwil{at}lsu.edu

A novel, Gram-negative bacterial strain, SUA2T, isolated from groundwater, was characterized using a polyphasic approach. Cells are Gram-negative, non-spore-forming, straight to curved rods with a single polar flagellum. Strain SUA2T is oxidase- and catalase-positive and is able to fix nitrogen. Poly-beta-hydroxybutyrate storage granules are produced. Dominant fatty acids when grown in R2A and VM ethanol media for 72 h at 37 °C are C16 : 0, C16 : 1{omega}7c, C17 : 0 cyclo, C10 : 0 3-OH, C18 : 1{omega}7c, C12 : 0 and C15 : 0. DNA G+C content is 67.9 mol%. Phenotypic and phylogenetic data indicate that strain SUA2T is related to, but clearly differentiated from Azospira oryzae. Strain SUA2T is thus proposed as a novel species of the genus Azospira with the name Azospira restricta sp. nov. The description of the genus Azospira is emended to include the characteristics of this novel species. The type strain of Azospira restricta is SUA2T (=NRRL B-41660T=DSM 18626T=LMG 23819T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and nifH sequences of strain SUA2T are DQ974114 and EF158046, respectively.

Supplementary figures showing morphological features and whole-cell soluble protein patterns (SDS-PAGE), and a supplementary table of fatty acid composition data are available with the online version of this paper.






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