HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lee, Y.-J. Articles by Wiegel, J. Search for Related Content PubMed PubMed Citation Articles by Lee, Y.-J. Articles by Wiegel, J. Agricola Articles by Lee, Y.-J. Articles by Wiegel, J.

Thermoanaerobacter sulfurigignens sp. nov., an anaerobic thermophilic bacterium that reduces 1 M thiosulfate to elemental sulfur and tolerates 90 mM sulfite

Yong-Jin Lee¹, Mona Dashti^1,, Alexander Prange^2,3, Fred A. Rainey⁴, Manfred Rohde⁵, William B. Whitman¹ and Juergen Wiegel¹

¹ Department of Microbiology, University of Georgia, Athens, GA 30602, USA
² Hochschule Niederrhein, FB Oecotrophologie, 41065 Mönchengladbach, Germany
³ Center for Advanced Microstructures and Devices (CAMD), Louisiana State University, Baton Rouge, LA 70806, USA
⁴ Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
⁵ Department of Microbial Pathogenicity, Helmholtz Center for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany

Correspondence
Juergen Wiegel
jwiegel{at}uga.edu

Two anaerobic thermophilic bacteria, designated strains JW/SL824 and JW/SL-NZ826^T, were isolated from an acidic volcanic steam outlet on White Island, New Zealand. Cells were rod-shaped, spore-forming, motile and Gram-stain negative, but contained Gram-type positive cell wall. Strain JW/SL-NZ826^T utilized various carbohydrates including xylose and glucose. The fermentation end products produced from glucose in the absence of thiosulfate were lactate, ethanol, acetate, CO₂ and H₂. The temperature range for growth was 34–72 °C, with an optimum at 63–67 °C. The pH^{60 °C} range for growth was 4.0–8.0, with an optimum at 5.0–6.5. The doubling time of strain JW/SL-NZ826^T under optimal growth conditions was 2.4 h. The DNA G+C content was 34–35 mol% (HPLC). The two strains reduced up to 1 M thiosulfate to elemental sulfur without sulfide formation, which is a trend typically observed among species belonging to the genus Thermoanaerobacterium. Sulfur globules containing short and long sulfur chains but no S₈-ring sulfur were produced inside and outside the cells. Up to 90 mM sulfite was tolerated. This tolerance is assumed to be an adaptation to the geochemistry of the environment of White Island. The 16S rRNA gene sequence analysis, however, indicated that the two strains belonged to the genus Thermoanaerobacter, with similarities in the range 95.6–92.7 %. Therefore, strains JW/SL-NZ824 and JW/SL-NZ826^T represent a novel taxon, for which the name Thermoanaerobacter sulfurigignens sp. nov. is proposed, with strain JW/SL-NZ826^T (=ATCC 700320^T=DSM 17917^T) as the type strain. Based on this and previous studies, an emended description of the genus Thermoanaerobacter is given.

Present address: Biological Sciences, Faculty of Sciences, University of Kuwait, Safat, Kuwait.

This article has been cited by other articles:

				I. D. Wagner, W. Zhao, C. L. Zhang, C. S. Romanek, M. Rohde, and J. Wiegel Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia Int J Syst Evol Microbiol, November 1, 2008; 58(11): 2565 - 2573. [Abstract] [Full Text] [PDF]

				Y.-J. Lee, R. I. Mackie, I. K. O. Cann, and J. Wiegel Description of Caldanaerobius fijiensis gen. nov., sp. nov., an inulin-degrading, ethanol-producing, thermophilic bacterium from a Fijian hot spring sediment, and reclassification of Thermoanaerobacterium polysaccharolyticum and Thermoanaerobacterium zeae as Caldanaerobius polysaccharolyticus comb. nov. and Caldanaerobius zeae comb. nov. Int J Syst Evol Microbiol, March 1, 2008; 58(3): 666 - 670. [Abstract] [Full Text] [PDF]

				Y.-J. Lee, A. Prange, H. Lichtenberg, M. Rohde, M. Dashti, and J. Wiegel In Situ Analysis of Sulfur Species in Sulfur Globules Produced from Thiosulfate by Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes J. Bacteriol., October 15, 2007; 189(20): 7525 - 7529. [Abstract] [Full Text] [PDF]