HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yoon, J. Articles by Yokota, A. Search for Related Content PubMed PubMed Citation Articles by Yoon, J. Articles by Yokota, A. Agricola Articles by Yoon, J. Articles by Yokota, A.

Pelagicoccus mobilis gen. nov., sp. nov., Pelagicoccus albus sp. nov. and Pelagicoccus litoralis sp. nov., three novel members of subdivision 4 within the phylum ‘Verrucomicrobia’, isolated from seawater by in situ cultivation

Jaewoo Yoon¹, Mina Yasumoto-Hirose^2,, Yoshihide Matsuo², Midori Nozawa², Satoru Matsuda², Hiroaki Kasai² and Akira Yokota¹

¹ Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan
² Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan

Correspondence
Jaewoo Yoon
aa57058{at}mail.ecc.u-tokyo.ac.jp

Five Gram-negative, white-pigmented, spherical, chemoheterotrophic bacteria were isolated from seawater from Japan and the Republic of Palau by use of an in situ cultivation technique. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the five novel isolates, 02PA-Ca-133^T, YM14-201^T, H-MN57^T, H-MN48 and MN1-156, were closely affiliated to members of subdivision 4 within the phylum ‘Verrucomicrobia’. The novel isolates shared 96–100 % sequence similarity with each other and showed less than 90 % similarity with the cultivated strains of subdivision 4. DNA–DNA relatedness values between strains 02PA-Ca-133^T, YM14-201^T and H-MN57^T were less than 70 %; the value commonly accepted as the threshold for the phylogenetic definition of a species. Antibiotic susceptibility tests and amino acid analysis of cell-wall hydrolysates indicated that the novel isolates did not contain muramic acid or diaminopimelic acid in their cell walls, suggesting that these strains lack peptidoglycan. The DNA G+C contents of the five strains were 51–57 mol%. The major menaquinone was MK-7 and C_{16 : 0}, C_{16 : 1}7c and anteiso-C_{15 : 0} were the major fatty acids. On the basis of polyphasic taxonomic evidence, it is concluded that these strains should be classified as representing a new genus and three novel species in subdivision 4 of the phylum ‘Verrucomicrobia’, for which the names Pelagicoccus mobilis gen. nov., sp. nov. [type strain 02PA-Ca-133^T (=MBIC08004^T=IAM 15422^T=KCTC 13126^T)], Pelagicoccus albus sp. nov. [type strain YM14-201^T (=MBIC08272^T=IAM 15421^T=KCTC 13124^T)] and Pelagicoccus litoralis sp. nov. [type strain H-MN57^T (=MBIC08273^T=IAM 15423^T=KCTC 13125^T)] are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 02PA-Ca-133^T, YM14-201^T and H-MN57^T are AB286015, AB286016 and AB286017, respectively.

Details of the composition of the ‘P’ medium used in the paper are available as supplementary material with the online version of this paper.

Present address: Okinawa Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST Okinawa Health Biotechnology Research Development Center, 12-75 Suzaki, Uruma Okinawa 904-2234, Japan.

This article has been cited by other articles:

				S. Mitra, Y. Matsuo, T. Haga, M. Yasumoto-Hirose, J. Yoon, H. Kasai, and A. Yokota Leptobacterium flavescens gen. nov., sp. nov., a marine member of the family Flavobacteriaceae, isolated from marine sponge and seawater Int J Syst Evol Microbiol, February 1, 2009; 59(2): 207 - 212. [Abstract] [Full Text] [PDF]

				J. Yoon, Y. Matsuo, S. Matsuda, K. Adachi, H. Kasai, and A. Yokota Rubritalea sabuli sp. nov., a carotenoid- and squalene-producing member of the family Verrucomicrobiaceae, isolated from marine sediment Int J Syst Evol Microbiol, April 1, 2008; 58(4): 992 - 997. [Abstract] [Full Text] [PDF]

				J. Yoon, Y. Matsuo, K. Adachi, M. Nozawa, S. Matsuda, H. Kasai, and A. Yokota Description of Persicirhabdus sediminis gen. nov., sp. nov., Roseibacillus ishigakijimensis gen. nov., sp. nov., Roseibacillus ponti sp. nov., Roseibacillus persicicus sp. nov., Luteolibacter pohnpeiensis gen. nov., sp. nov. and Luteolibacter algae sp. nov., six marine members of the phylum 'Verrucomicrobia', and emended descriptions of the class Verrucomicrobiae, the order Verrucomicrobiales and the family Verrucomicrobiaceae Int J Syst Evol Microbiol, April 1, 2008; 58(4): 998 - 1007. [Abstract] [Full Text] [PDF]

				J. Yoon, N. Oku, S. Matsuda, H. Kasai, and A. Yokota Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum 'Verrucomicrobia' isolated from seagrass Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2874 - 2880. [Abstract] [Full Text] [PDF]

				J. Yoon, Y. Matsuo, S. Matsuda, K. Adachi, H. Kasai, and A. Yokota Rubritalea spongiae sp. nov. and Rubritalea tangerina sp. nov., two carotenoid- and squalene-producing marine bacteria of the family Verrucomicrobiaceae within the phylum 'Verrucomicrobia', isolated from marine animals Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2337 - 2343. [Abstract] [Full Text] [PDF]

				A. Kageyama, Y. Takahashi, Y. Matsuo, H. Kasai, Y. Shizuri, and S. Omura Microbacterium sediminicola sp. nov. and Microbacterium marinilacus sp. nov., isolated from marine environments Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2355 - 2359. [Abstract] [Full Text] [PDF]

				J. Yoon, Y. Matsuo, S. Matsuda, K. Adachi, H. Kasai, and A. Yokota Cerasicoccus arenae gen. nov., sp. nov., a carotenoid-producing marine representative of the family Puniceicoccaceae within the phylum 'Verrucomicrobia', isolated from marine sand Int J Syst Evol Microbiol, September 1, 2007; 57(9): 2067 - 2072. [Abstract] [Full Text] [PDF]