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Aquincola tertiaricarbonis gen. nov., sp. nov., a tertiary butyl moiety-degrading bacterium

Ute Lechner¹, Danny Brodkorb¹, Roland Geyer^3,, Gerd Hause², Claus Härtig³, Georg Auling⁴, Françoise Fayolle-Guichard⁵, Pascal Piveteau⁵, Roland H. Müller³ and Thore Rohwerder^3,

¹ Institut für Mikrobiologie, Kurt-Mothes-Str. 3, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany
² Biozentrum, Weinbergweg 22, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany
³ Umweltforschungszentrum Leipzig-Halle, Department Umweltmikrobiologie, Permoserstr. 15, D-04318 Leipzig, Germany
⁴ Institut für Mikrobiologie, Universität Hannover, Schneiderberg 50, D-30167 Hannover, Germany
⁵ Institute Français du Pétrole, 1-4, avenue de Bois-Préau, 92852 Rueil-Malmaison, France

Correspondence
Ute Lechner
ute.lechner{at}mikrobiologie.uni-halle.de

Strains L10^T, L108 and CIP I-2052 were originally obtained from methyl tert-butyl ether (MTBE)-contaminated groundwater and from a wastewater treatment plant, respectively. All share the ability to grow on tert-butanol, an intermediate of MTBE degradation. Cells are strictly aerobic, motile by a polar flagellum and exhibit strong pili formation. Poly -hydroxybutyrate (PHB) granules are formed. The DNA G+C content is 69–70.5 mol% and the main ubiquinone is Q-8. The major cellular fatty acids are 16 : 1 cis-9 and 16 : 0 and the only hydroxy fatty acid is 10 : 0 3-OH. The major phospholipids are phosphatidylethanolamine (PE) 16 : 1/16 : 1 and phosphatidylglycerol 16 : 0/16 : 1. A significant amount of PE 17 : 0/16 : 1 is present. The 16S rRNA gene sequences of these strains are almost identical and form a separate line of descent in the Rubrivivax–Roseateles–Leptothrix–Ideonella–Aquabacterium branch of the Betaproteobacteria with 97 % similarity to 16S rRNA genes of the type strains of Rubrivivax gelatinosus, Leptothrix mobilis and Ideonella dechloratans. However, physiological properties, DNA–DNA relatedness values and the phospholipid and cellular fatty acid profiles distinguish the novel isolates from the three closely related genera. Therefore, it is concluded that strains L10^T, L108 and CIP I-2052 represent a new genus and novel species for which the name Aquincola tertiaricarbonis gen. nov., sp. nov., is proposed. The type strain is strain L10^T (=DSM 18512^T=CIP 109243^T).

The GenBank/EMBL/DDBJ accession number for the partial 16S rRNA gene sequence of strain L10^T is DQ656489.

Present address: Applera Europe B.V., LC-MS Support, Grundstrasse 10, 6343 Rotkreuz, Switzerland.

Present address: Aquatische Biotechnologie, Biofilm-Zentrum, Universität Duisburg-Essen, Geibelstr. 41, D-47057 Duisburg, Germany.

This article has been cited by other articles:

				R. H. Muller, T. Rohwerder, and H. Harms Degradation of fuel oxygenates and their main intermediates by Aquincola tertiaricarbonis L108 Microbiology, May 1, 2008; 154(5): 1414 - 1421. [Abstract] [Full Text] [PDF]