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Chimaereicella boritolerans sp. nov., a boron-tolerant and alkaliphilic bacterium of the family Flavobacteriaceae isolated from soil

¹ Biotechnology Research Center, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan
² National Agricultural Research Centre, Park Road, Islamabad-45500, Pakistan
³ Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan
⁴ SORST, JST, Chiyoda-Ku, Tokyo, Japan

Correspondence
Toru Fujiwara
atorufu{at}mail.ecc.u-tokyo.ac.jp
Iftikhar Ahmed
iftikharnarc{at}hotmail.com

A non-motile, Gram-negative, boron-tolerant and alkaliphilic bacterium was isolated from soil of the Hisarcik area in the Kutahya Province of Turkey that was naturally high in boron minerals. The novel isolate, designated T-22^T, formed rod-shaped cells, was catalase- and oxidase-positive and tolerated up to 300 mM boron. The strain also survived on agar medium containing up to 3 % (w/v) NaCl. The pH range for growth of this strain was 6.5–10.0 (optimum pH 8.0–9.0) and the temperature range was 16–37 °C (optimum 28–30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed a clear affiliation with the genus Chimaereicella, with 97.4 % sequence similarity to Chimaereicella alkaliphila AC-74^T, which was the highest similarity among cultivated bacteria. The DNA–DNA relatedness with C. alkaliphila AC-74^T was 28.3 %. The major respiratory quinone system was MK-7 and the predominant cellular fatty acids were iso-C_{15 : 0}, iso-C_{17 : 1}9c, iso-C_{17 : 0} 3-OH and summed feature 3 (iso-C_{15 : 0} 2-OH and/or iso-C_{16 : 1}7c). The DNA G+C content was 42.5 mol%. Based on the phylogenetic analysis and physiological, chemotaxonomic and genetic data, we concluded that strain T-22^T should be classified in the genus Chimaereicella, and we propose the name Chimaereicella boritolerans sp. nov. for this novel species. The type strain is strain T-22^T (=DSM 17298^T=NBRC 101277^T=ATCC BAA-1189^T).

Maximum-likelihood and maximum-parsimony trees based on 16S rRNA gene sequence data are available as supplementary material in IJSEM Online.

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