HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, L. Articles by Patel, B. K. C. Search for Related Content PubMed PubMed Citation Articles by Zhang, L. Articles by Patel, B. K. C. Agricola Articles by Zhang, L. Articles by Patel, B. K. C.

Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage

¹ Microbial Gene Research and Resources Facility, School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia
² Center for Forestry and Horticultural Research and Australian School of Environmental Studies, Faculty of Environmental Sciences, Griffith University, Brisbane, QLD 4111, Australia

Correspondence
Bharat K. C. Patel
b.patel{at}griffith.edu.au

An aerobic bacterium, designated strain E5HC-32^T, was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8–1.6x2.6–4.8 µm) produced subterminal spherical spores which distended the cells. Strain E5HC-32^T grew optimally in 1 % trypticase soy broth (TSB) at 30 °C (temperature range for growth, 25–40 °C) and a pH of 8.4 (pH growth range, pH 7.1–9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections showed the presence of a typical Gram-positive type cell-wall ultrastructure. The strain was catalase-positive and oxidase-negative and metabolized pyruvic acid methyl ester, D-galactonic acid lactone, -ketobutyric acid, -ketovaleric acid, L-proline, L-alanine, urocanic acid, inosine, uridine, thymidine, glycerol, -cyclodextrin, -D-lactose, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, turanose, cis-aconitic acid, -hydroxybutyric acid, L-alaninamide and 2-aminoethanol. The G+C content of DNA was 41±1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis revealed that strain E5HC-32^T was placed equidistantly as a member of the class Bacilli, phylum Firmicutes, with Bacillus sphaericus DSM 28^T and Bacillus odysseyi ATCC PTA-4993^T (similarity of 93 %). In addition to its significant phylogenetic separation from its nearest relatives, strain E5HC-32^T possessed phenotypic traits that also suggested that it represented a novel species, for which the name Bacillus decisifrondis sp. nov. is proposed. The type strain is E5HC-32^T (=JCM 13601^T=DSM 17725^T).

Transmission electron micrographs of cells of strain E5HC-32^T are available as a supplementary figure in IJSEM Online.

This article has been cited by other articles:

				A. E. Perkins and W. L. Nicholson Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants J. Bacteriol., February 1, 2008; 190(3): 807 - 814. [Abstract] [Full Text] [PDF]