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Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces

Hidenori Hayashi^1,, Kensaku Shibata^1,2, Mitsuo Sakamoto¹, Shinichi Tomita² and Yoshimi Benno¹

¹ Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
² Department of Life Science, Faculty of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, Japan

Correspondence
Hidenori Hayashi
h-hayashi{at}maebashi-it.ac.jp

Six strains (CB7^T, CB18, CB23, CB26, CB28 and CB35^T) were isolated from human faeces. Based on phylogenetic analysis, phenotypic characteristics, cellular fatty acid profiles and menaquinone profiles, these strains could be included within the genus Prevotella and made up two clusters. 16S rRNA gene sequence analysis indicated that five strains were most closely related to Prevotella veroralis, sharing about 92 % sequence similarity; the remaining strain was most closely related to Prevotella shahii, sharing about 90 % sequence similarity. All six strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid compositions of the six strains differed significantly from those of other Prevotella species. Five strains (CB7^T, CB18, CB23, CB26 and CB28) contained dimethyl acetals and the major menaquinones of these strains were MK-11, MK-12 and MK-13. The major menaquinones of CB35^T were MK-12 and MK-13. Based on phenotypic and phylogenetic findings, two novel species, Prevotella copri sp. nov. and Prevotella stercorea sp. nov., are proposed, representing the two different strain clusters. The DNA G+C contents of strains CB7^T and CB35^T were 45.3 and 48.2 mol%, respectively. The type strains of P. copri and P. stercorea are CB7^T (=JCM 13464^T=DSM 18205^T) and CB35^T (=JCM 13469^T=DSM 18206^T), respectively.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of P. copri CB7^T and P. stercorea CB35^T are AB064923 and AB244774, respectively.

API 20A test results, cellular fatty acid compositions, menaquinone compositions and API ZYM and API An-Ident test results of P. copri sp. nov., P. stercorea sp. nov. and related species are available as supplementary material in IJSEM Online.

Present address: Faculty of Engineering, Maebashi Institute of Technology, 460-1 Kamisatori, Maebashi, Gunma 371-0816, Japan.

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