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Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives

Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain

Correspondence
Mercedes Monteoliva-Sánchez
mmonteol{at}ugr.es

Four bacterial strains (E₃₀8^T, E₅₅49, I₃₀77 and N₃₀129) were isolated from the residual wash-water produced during the processing of Spanish-style green table olives. The isolates were subjected to a polyphasic taxonomic study using phenotypic, phylogenetic and genotypic methods. The bacteria were Gram-positive, spore-forming rods. Moreover, they were heterotrophs that were able to utilize cellobiose, glucose, mannose and rhamnose as carbon sources. The G+C content of their genomic DNA ranged from 30.7 to 33.4 mol%. The major cellular fatty acids found in strain E₃₀8^T were iso-C_{15 : 0}, anteiso-C_{15 : 0}, iso-C_{17 : 0} and anteiso-C_{17 : 0}. DNA–DNA hybridization shows 76.2–88.3 % relatedness among the four strains. The 16S rRNA gene sequence of isolate E₃₀8^T shows that it belongs to the genus Virgibacillus, with the highest sequence similarity (99 %) to Virgibacillus marismortui 123^T. However, phenotypic differences and DNA–DNA relatedness between strain E₃₀8^T and V. marismortui ATCC 700626^T of less than 47 % suggest the placement of these strains within a novel species of the genus Virgibacillus. The name Virgibacillus olivae sp. nov. is proposed, with strain E₃₀8^T (=LMG 23503^T=DSM 18098^T) as the type strain.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence of strain E₃₀8^T is DQ139839.

A 16S rRNA gene sequence-based maximum-parsimony tree is available as supplementary material in IJSEM Online.

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