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Int J Syst Evol Microbiol 57 (2007), 1143-1147; DOI  10.1099/ijs.0.64668-0
© 2007 International Union of Microbiological Societies

Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China

Qian-fu Wang, Wei Li, Yan-li Liu, Hai-hua Cao, Zhao Li and Guang-qin Guo

Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China

Correspondence
Guang-Qin Guo
gqguo{at}lzu.edu.cn

A moderately haloalkaliphilic, Gram-positive bacterium, designated as strain CM1T, was isolated from a crude sea-salt sample collected near Qingdao in eastern China. Strain CM1T was found to grow optimally at 37 °C and pH 9.0. It was shown to be aerobic, rod-shaped and capable of growth at salinities of 2.5–20 % (w/v) NaCl (optimum, 12 %). The genomic DNA G+C content was about 48 mol%. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 and the major isoprenoid quinones were MK-7(H2) and MK-6(H2). Phylogenetic analyses based on 16S rRNA gene sequences revealed that CM1T is a member of the genus Bacillus and has less than 95.2 % gene sequence similarity to the most closely related strain, Bacillus salarius BH169T. Its DNA–DNA reassociation value with respect to B. salarius BH169T was 35.4 %. On the basis of phenotypic and molecular properties, strain CM1T represents a novel Bacillus species, for which the name Bacillus qingdaonensis sp. nov. is proposed. The type strain is CM1T (=CGMCC 1.6134T=JCM 14087T).


The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CM1T is DQ115802.

Phylogenetic trees based on maximum-parsimony and minimum-evolution analyses of the 16S rRNA gene sequences of strain CM1T and related species are available as supplementary figures in IJSEM Online.







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Copyright © 2007 by the International Union of Microbiological Societies.