| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Biotechnology Research Center, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
2 National Agricultural Research Centre, Park Road, Islamabad – 45500, Pakistan
3 Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
4 Research Center for Water Environmental Technology, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8657, Japan
5 SORST, JST, Chiyoda-ku, Tokyo, Japan
Correspondence
Iftikhar Ahmed
iftikharnarc{at}hotmail.com
Toru Fujiwara
atorufu{at}mail.ecc.u-tokyo.ac.jp
Three strains of a spore-forming, Gram-positive, motile, rod-shaped and boron-tolerant bacterium were isolated from soil. The strains, designated 10aT, 11c and 12B, can tolerate 5 % (w/v) NaCl and up to 150 mM boron, but optimal growth was observed without addition of boron or NaCl in Luria–Bertani agar medium. The optimum temperature for growth was 37 °C (range 16–45 °C) and the optimum pH was 7.0–8.0 (range pH 5.5–9.5). A comparative analysis of the 16S rRNA gene sequence demonstrated that the isolated strains were closely related to Bacillus fusiformis DSM 2898T (97.2 % similarity) and Bacillus sphaericus DSM 28T (96.9 %). DNA–DNA relatedness was greater than 97 % among the isolated strains and 61.1 % with B. fusiformis DSM 2898T and 43.2 % with B. sphaericus IAM 13420T. The phylogenetic and phenotypic analyses and DNA–DNA relatedness indicated that the three strains belong to the same species, that was characterized by a DNA G+C content of 36.5–37.9 mol%, MK-7 as the predominant menaquinone system and iso-C15 : 0 (32 % of the total) as a major cellular fatty acid. In contrast to the type species of the genus Bacillus, the strains contained peptidoglycan with lysine, aspartic acid, alanine and glutamic acid. Based on the distinctive peptidoglycan composition, phylogenetic analyses and physiology, the strains are assigned to a novel species within a new genus, for which the name Lysinibacillus boronitolerans gen. nov., sp. nov. is proposed. The type strain of Lysinibacillus boronitolerans is strain 10aT (=DSM 17140T=IAM 15262T=ATCC BAA-1146T). It is also proposed that Bacillus fusiformis and Bacillus sphaericus be transferred to this genus as Lysinibacillus fusiformis comb. nov. and Lysinibacillus sphaericus comb. nov., respectively.
Results of growth experiments in the presence of boron, photomicrographs of cells of strain 10aT and results of separation of polar lipids of strain 10aT and related type strains are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  H. Miwa, I. Ahmed, J. Yoon, A. Yokota, and T. Fujiwara Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil Int J Syst Evol Microbiol, January 1, 2008; 58(1): 286 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Albert, J. Archambault, M. Lempa, B. Hurst, C. Richardson, S. Gruenloh, M. Duran, H. L. Worliczek, B. E. Huber, R. Rossello-Mora, et al. Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov. Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2729 - 2737. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
