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Int J Syst Evol Microbiol 57 (2007), 866-869; DOI  10.1099/ijs.0.64750-0
© 2007 International Union of Microbiological Societies

Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier

De-Chao Zhang1,2, He-Xiang Wang2, Heng-Lin Cui1, Yong Yang1, Hong-Can Liu1, Xiu-Zhu Dong1 and Pei-Jin Zhou1

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
2 State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China

Correspondence
Pei-Jin Zhou
zhou{at}sun.im.ac.cn

A novel psychrotolerant, Gram-positive, yellow-pigmented, aerobic bacterium, strain 0549T, was isolated from the China No. 1 glacier. The cells of the isolate were catalase-positive, motile, irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain 0549T was able to grow at 4–27 °C, with optimum growth occurring at 20–22 °C. The major fatty acids were anteiso-C15 : 0, anteiso-C15 : 1, iso-C16 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 67 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 0549T belonged to the genus Cryobacterium and exhibited 96.5 % similarity to Cryobacterium psychrophilum JCM 1463T. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties and the DNA–DNA relatedness data, strain 0549T represents a novel species of the genus Cryobacterium, for which the name Cryobacterium psychrotolerans is proposed. The type strain is 0549T (=CGMCC 1.5382T=JCM 13925T).


The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 0549T is DQ515963.

The fatty acid profiles of strain 0549T and Cryobacterium psychrophilum JCM 1463T are available in a supplementary table in IJSEM Online.




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