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Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain

José Luis Zurdo-Piñeiro¹, Raúl Rivas^1,, Martha E. Trujillo¹, Nieves Vizcaíno¹, José Antonio Carrasco², Manuel Chamber², Antonio Palomares³, Pedro F. Mateos¹, Eustoquio Martínez-Molina¹ and Encarna Velázquez¹

¹ Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain
² CIFA Las Torres, Alcalá del Río, Sevilla, Spain
³ Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain

Correspondence
Encarna Velázquez
evp{at}usal.es

Two strains named ESC1^T and ESC5 were isolated from nodules of Cytisus scoparius growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene showed that these strains belong to the genus Ochrobactrum, their closest relatives being Ochrobactrum anthropi and Ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. Despite this high similarity, the results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a novel species of genus Ochrobactrum. The DNA–DNA hybridization values were respectively 70, 66 and 55 % with respect to O. lupini LUP21^T, O. anthropi DSM 6882^T and Ochrobactrum tritici DSM 13340^T. The predominant fatty acids were C_{18 : 1}7c and C_{18 : 1} 2-OH. Strains ESC1^T and ESC5 were strictly aerobic and were able to reduce nitrate and to hydrolyse aesculin. They produced -galactosidase and -glucosidase and did not produce urease after 48 h incubation. The G+C content of strain ESC1^T was 56.4 mol%. Both strains ESC1^T and ESC5 contained nodD and nifH genes on megaplasmids that were related phylogenetically to those of rhizobial strains nodulating Phaseolus, Leucaena, Trifolium and Lupinus. From the results of this work, we propose that the strains isolated in this study be included in a novel species named Ochrobactrum cytisi sp. nov. The type strain is ESC1^T (=LMG 22713^T=CECT 7172^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, nifH and nodD gene sequences of strain ESC1^T are AY776289–AY776291, respectively.

TP-RAPD profiles, nifH- and nodD-based neighbour-joining trees, photos of nodules induced by strain ESC1^T and R. etli CFN42^T, fatty acid profiles and differentiating characters for all species of Ochrobactrum are available as supplementary material in IJSEM Online.

Present address: Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.

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