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Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan

¹ Department of Applied Chemistry, Faculty of Engineering, and Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan
² Halophiles Research Institute, 677-1 Shimizu, Noda, Chiba 278-0043, Japan

Correspondence
Akinobu Echigo
dc0400017{at}toyonet.toyo.ac.jp

Two alkaliphilic, moderately halophilic bacteria, designated BM2^T and HN2, were isolated from non-saline forest soil in Japan. The cells of strain BM2^T were motile, aerobic, rod-shaped and Gram-positive. The peptidoglycan was of the A1 type, and the diamino acid was meso-diaminopimelic acid. Growth was observed at NaCl concentrations between 5.0 and 25.0 % (w/v) (the optimum being 10.0–15.0 %, w/v), at pH 7.0–10.0 (optimum, pH 9.0–9.5) and at 20–50 °C. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C_{15 : 0} and anteiso-C_{17 : 0}. The G+C content of total DNA of strain BM2^T was 37.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain BM2^T was most closely related to Alkalibacillus haloalkaliphilus DSM 5271^T (98.0 % sequence similarity). DNA–DNA hybridization results indicated low levels of relatedness between strain BM2^T and A. haloalkaliphilus JCM 12303^T (23 and 16 % reciprocally), Alkalibacillus filiformis JCM 13893^T (25 and 21 %) and Alkalibacillus salilacus JCM 13894^T (27 and 19 %). On the basis of the phylogenetic and phenotypic characteristics, strain BM2^T represents a novel species, for which the name Alkalibacillus silvisoli sp. nov. is proposed. The type strain is BM2^T (=JCM 14193^T=DSM 18495^T).

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