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Int J Syst Evol Microbiol 57 (2007), 745-750; DOI  10.1099/ijs.0.64700-0
© 2007 International Union of Microbiological Societies

Lactobacillus secaliphilus sp. nov., isolated from type II sourdough fermentation

Matthias A. Ehrmann1, Markus Brandt2, Peter Stolz2, Rudi F. Vogel1 and Maher Korakli1

1 Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, D-85350 Freising, Germany
2 Ernst BÖCKER GmbH & Co. KG Postfach 21, D-32378 Minden, Germany

Correspondence
Matthias A. Ehrmann
M.Ehrmann{at}wzw.tum.de

Two strains of Gram-positive, catalase-negative, lactic acid bacteria, strains TMW 1.1309T and TMW 1.1313, were isolated at an interval of several years from an industrial type II sourdough. They occurred at cell numbers of 8x108 c.f.u. g–1 and therefore were considered to be one of the dominant members of the microbiota in this type of fermentation. Cells of both strains grow exclusively on modified MRS containing trypsin-digested rye-bran extract. Both strains possessed identical 16S rRNA gene sequences, but could be discriminated by RAPD fingerprints. Comparative 16S rRNA and tuf gene sequence analyses positioned strain TMW 1.1309T as part of the Lactobacillus reuteri phylogenetic group within the genus Lactobacillus. The 16S rRNA gene sequence similarities to the closest related species, Lactobacillus coleohominis and Lactobacillus ingluviei were 97.1 and 95.4 %, respectively. The DNA G+C content of strain TMW 1.1309T was 48 mol%. Growth characteristics, biochemical features and DNA–DNA hybridization values below 70 % with all the nearest neighbours demonstrated that the isolates represent a novel Lactobacillus species. The name Lactobacillus secaliphilus sp. nov. is proposed for the novel isolates, with the type strain TMW 1.1309T (=DSM 17896T=CCUG 53218T).

Abbreviations: RAPD, randomly amplified polymorphic DNA

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains TMW 1.1309T and TMW 1.1313 are AM279150 and AM411002, respectively. The GenBank/EMBL/DDBJ accession numbers for the tuf gene sequences of L. mucosae DSM 13345T, L. coleohominis CCUG 44007T, L. secaliphilus DSM 17896T, L. ingluviei DSM15946T, L. cellobiosus DSM 20055T, L. pontis DSM 8475T, L. frumenti DSM 13145T, L. panis DSM 6035T and L. oris DSM 4864T are AM295057–AM295065, respectively.

Additional phylogenetic trees based on maximum-likelihood and maximum-parsimony analyses of the 16S rRNA gene sequence of strain TMW 1.1309T are available as supplementary figures in IJSEM Online.






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