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Granulicoccus phenolivorans gen. nov., sp. nov., a Gram-positive, phenol-degrading coccus isolated from phenol-degrading aerobic granules

Abdul Majid Maszenan¹, He Long Jiang¹, Joo-Hwa Tay¹, Peter Schumann², Reiner M. Kroppenstedt² and Stephen Tiong-Lee Tay^1,

¹ Environmental Engineering Research Centre, School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798
² DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7b, D-38124 Braunschweig, Germany

Correspondence
Abdul Majid Maszenan
cmaszenan{at}ntu.edu.sg

A Gram-positive bacterium, designated strain PG-02^T, was isolated by serial dilution from aerobic granules obtained from a laboratory-scale sequencing batch reactor for bioremediation of phenolic wastewater. Strain PG-02^T grew axenically as cocci and is an oxidase-negative and catalase-positive, non-motile facultative anaerobe. It does not reduce nitrate and grows between 15 and 37 °C, with an optimum temperature of 30 °C. The pH range for growth is between 5.0 and 8.5, with an optimum pH of 7.0. Strain PG-02^T contains type A3 peptidoglycan (LL-A₂pmGly with alanine at position 1 of the peptide subunit). The G+C content of the DNA is 69 mol%. Menaquinone MK-9(H₄) was the major isoprenoid quinone. The polar lipids included diphosphatidylglycerol and phosphatidylglycerol, while 13-methyltetradecanoic acid (i-C_{15 : 0}) and 1,1-dimethoxy-iso-pentadecane (i-C_{15 : 0} DMA) were the major components in whole-cell methanolysates. PG-02^T stained positively for intracellular polyphosphate granules but not poly--hydroxyalkanoates. It produces capsular material and possesses an autoaggregation capability. Phenotypic and 16S rRNA gene sequence analyses showed that PG-02^T differed from its closest phylogenetic relatives, namely members of the suborder Propionibacterineae, which includes the genera Tessaracoccus, Microlunatus, Luteococcus, Micropruina, Propionibacterium, Propioniferax, Nocardioides, Friedmanniella and Aeromicrobium, and that it should be placed in a new genus and species as Granulicoccus phenolivorans gen. nov., sp. nov. The type strain of Granulicoccus phenolivorans is PG-02^T (=ATCC BAA-1292^T=DSM 17626^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PG-02^T is AY566575.

Details of signature nucleotides within the 16S rRNA gene sequences of strain PG-02^T and related taxa are available as supplementary material in IJSEM Online.

Deceased 29 July 2005.

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