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Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell

Dawn E. Holmes^1,, Kelly P. Nevin^1,, Trevor L. Woodard¹, Aaron D. Peacock² and Derek R. Lovley¹

¹ Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
² The University of Tennessee Center for Biomarker Analysis, 10515 Research Drive, Suite 300, Knoxville, TN 37932-2575, USA

Correspondence
Dawn E. Holmes
dholmes{at}microbio.umass.edu

A Gram-negative, non-motile, filamentous, rod-shaped, non-spore-forming bacterium (strain F2^T) was isolated from the surface of an electricity-harvesting electrode incubated in marine sediments. Strain F2^T does not contain c-type cytochromes, flexirubin or carotenoids. It is a facultative anaerobe that can ferment sugars by using a mixed acid fermentation pathway and it can grow over a wide range of temperatures (4–42 °C). The DNA G+C (44.9 mol%) content and chemotaxonomic characteristics (major fatty acids, a-15 : 0 and 15 : 0) were consistent with those of species within the phylum Bacteroidetes. Phylogenetic analysis of the 16S rRNA nucleotide and elongation factor G amino acid sequences indicated that strain F2^T represents a unique phylogenetic cluster within the phylum Bacteroidetes. On the basis of 16S rRNA gene sequence phylogeny, the closest relative available in pure culture, Alkaliflexus imshenetskii, is only 87.5 % similar to strain F2^T. Results from physiological, biochemical and phylogenetic analyses showed that strain F2^T should be classified as a novel genus and species within the phylum Bacteroidetes, for which the name Prolixibacter bellariivorans gen. nov., sp. nov. is proposed. The type strain is F2^T (=ATCC BAA-1284^T=JCM 13498^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and fusA gene sequences of strain F2^T are AY918928 and AY918929, respectively.

The phospholipid fatty acid content (%) of strain F2^T and a 16S rRNA gene sequence similarity matrix for strain F2^T and other Bacteroidetes species are available in supplementary tables in IJSEM Online.

These authors contributed equally to this work.